RAB33A Polyclonal antibody

RAB33A Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11025-1-AP

Synonyms

RAB33A, RabS10, Ras related protein Rab 33A, Small GTP binding protein S10

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Validation Data Gallery

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  WB analysis of mouse brain using 11025-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11025-1-AP (RAB33A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11025-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11025-1-AP (RAB33A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 11025-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11025-1-AP (RAB33A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 11025-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11025-1-AP (RAB33A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 11025-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11025-1-AP (RAB33A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 11025-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11025-1-AP (RAB33A antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of PC-12 using 11025-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using RAB33A antibody (11025-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue
• Positive IHC detected in: human brain tissue, human gliomas tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

11025-1-AP targets RAB33A in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB33A fusion protein Ag1492
• Full Name: RAB33A, member RAS oncogene family
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC009996
• Gene Symbol: RAB33A
• Gene ID (NCBI): 9363
• RRID: AB_2238178
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB, IF	Biochem Biophys Res Commun; The small GTPase Rab33A participates in regulation of amylase release from parotid acinar cells.; Authors - Akane Imai