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  • KD/KO Validated

RAB35 Polyclonal antibody

RAB35 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 11329-2-AP

Synonyms

GTP binding protein RAY, H ray, RAB1C, RAB35, Ras related protein Rab 1C, Ras related protein Rab 35, RAY



Tested Applications

Positive WB detected inA431 cells, human brain tissue, A375 cells, HeLa cells, MCF-7 cells, RAW 264.7 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11329-2-AP targets RAB35 in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity canine, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB35 fusion protein Ag1872
Full Name RAB35, member RAS oncogene family
Calculated molecular weight 201 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC015931
Gene symbol RAB35
Gene ID (NCBI) 11021
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB35 is a member of the small GTPase superfamily. Like the other Rab proteins, RAB35 has 2 consecutive cysteine residues at its C terminus, and this region is thought to be involved in membrane association. Rab35 is found on the cell surface and endosomes, where it has been proposed to play a role in recycling of the transferrin and other receptors.

Protocols

Product Specific Protocols
WB protocol for RAB35 antibody 11329-2-APDownload protocol
IHC protocol for RAB35 antibody 11329-2-APDownload protocol
IF protocol for RAB35 antibody 11329-2-APDownload protocol
IP protocol for RAB35 antibody 11329-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.

Authors - Seiichi Koike
ratWB

Adv Healthc Mater

Improved Small Extracellular Vesicle Secretion of Rat Adipose-Derived Stem Cells by Microgrooved Substrates through Upregulation of the ESCRT-III-Associated Protein Alix.

Authors - Yuyong Ji
humanIF

Cell Struct Funct

Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35.

Authors - Fukuda Mitsunori M
mouseIF

Histochem Cell Biol

Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation.

Authors - H H Wang
canineWB,IF

J Biol Chem

Rab35-GEFs, DENND1A and folliculin differentially regulate podocalyxin trafficking in two- and three-dimensional epithelial cell cultures.

Authors - Riko Kinoshita
  • KO Validated
humanWB

J Biol Chem

Rab35 Regulates Evoked Exocytosis of Endothelial Weibel-Palade Bodies.

Authors - Anja Biesemann
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tom (Verified Customer) (02-24-2021)

HEK293T whole cell lysate probed with Rab35 antibody overnight at 4 degrees. Membrane was blocked in 5% BSA for one hour prior to incubation with antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HEK293T
RAB35 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:11329-2-AP)
FH

Mayur (Verified Customer) (01-14-2019)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse brain tissue (Cortex)
RAB35 Antibody Western Blot, validation (1:1000 dilution) in Mouse brain tissue (Cortex) (Cat no:11329-2-AP)