|Positive WB detected in||A431 cells, human brain tissue, A375 cells, HeLa cells, MCF-7 cells, RAW 264.7 cells, mouse brain tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human breast cancer tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11329-2-AP targets RAB35 in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||canine, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RAB35 fusion protein Ag1872|
|Full Name||RAB35, member RAS oncogene family|
|Calculated molecular weight||201 aa, 23 kDa|
|Observed molecular weight||23 kDa|
|GenBank accession number||BC015931|
|Gene ID (NCBI)||11021|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RAB35 is a member of the small GTPase superfamily. Like the other Rab proteins, RAB35 has 2 consecutive cysteine residues at its C terminus, and this region is thought to be involved in membrane association. Rab35 is found on the cell surface and endosomes, where it has been proposed to play a role in recycling of the transferrin and other receptors.
|Product Specific Protocols|
|WB protocol for RAB35 antibody 11329-2-AP||Download protocol|
|IHC protocol for RAB35 antibody 11329-2-AP||Download protocol|
|IF protocol for RAB35 antibody 11329-2-AP||Download protocol|
|IP protocol for RAB35 antibody 11329-2-AP||Download protocol|
|Click here to view our Standard Protocols|
SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Cell Struct Funct
Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35.
Histochem Cell Biol
Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation.
J Biol Chem
Rab35-GEFs, DENND1A and folliculin differentially regulate podocalyxin trafficking in two- and three-dimensional epithelial cell cultures.
J Biol Chem
Rab35 Regulates Evoked Exocytosis of Endothelial Weibel-Palade Bodies.
Rab35 is required for Wnt5a/Dvl2-induced Rac1 activation and cell migration in MCF-7 breast cancer cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tom (Verified Customer) (02-24-2021)
HEK293T whole cell lysate probed with Rab35 antibody overnight at 4 degrees. Membrane was blocked in 5% BSA for one hour prior to incubation with antibody.
Mayur (Verified Customer) (01-14-2019)