RAB37 Polyclonal antibody

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• RAB37 Polyclonal antibody (13051-1-AP)

RAB37 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13051-1-AP Copied!

Publications (10)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

Ras related protein Rab 37, Ras-related protein Rab-37

• 

  WB analysis using 13051-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13051-1-AP (RAB37 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 13051-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13051-1-AP (RAB37 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 13051-1-AP

  IP result of anti-RAB37 (IP:13051-1-AP, 4ug; Detection:13051-1-AP 1:3000) with mouse brain tissue lysate 1280 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 13051-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13051-1-AP (RAB37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 13051-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13051-1-AP (RAB37 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse spleen tissue using 13051-1-AP

  IHC result from Barry Bradford of 13051-1-AP (1:1,000) in Mouse Spleen.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human lung cancer tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13051-1-AP targets RAB37 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3717

  Product name: Recombinant human RAB37 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-223 aa of BC016615

  Sequence: MTGTPGAVATRDGEAPERSPPCSPSYDLTGKVMLLGDTGVGKTCFLIQFKDGAFLSGTFIATVGIDFRNKVVTVDGVRVKLQIWDTAGQERFRSVTHAYYRDAQALLLLYDITNKSSFDNIRAWLTEIHEYAQRDVVIMLLGNKADMSSERVIRSEDGETLAREYGVPFLETSAKTGMNVELAFLAIAKELKYRAGHQADEPSFQIRDYVESQKKRSSCCSFM

• Full Name: RAB37, member RAS oncogene family
• Calculated Molecular Weight: 223 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC016615
• Gene Symbol: RAB37
• Gene ID (NCBI): 326624
• RRID: AB_10642695
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96AX2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB37, as a small GTPase, plays important roles in several cellular processes through intracellular membrane traffic. RAB37 can regulate insulin secretion in β-cells 7 and TNF-α secretion from activated macrophages. In allergic responses, RAB37 regulated mast cell degranulation. RAB37 has been identified to be a tumor suppressor and it regulated exocytosis of several proteins including TIMP metallopeptidase inhibitor 1 (TIMP1), secreted frizzled-related protein-1 (SFRP1) 12, thrombospondin-1 (TSP1), and soluble ST2.

Publications

What published studies show

RAB37 Polyclonal antibody (13051-1-AP) has 10 total citations published in Molecular Cancer, Autophagy, Nature Communications, Cell Death & Differentiation, Clinical Cancer Research, Cell Reports, Cell Oncology, and Kaohsiung Journal of Medical Sciences. Associated research fields include autophagy regulation, cancer metastasis (colon, gastric, nasopharyngeal), insulin secretion, ovarian homeostasis, retinal function, viral protein transport, exosome release, and epithelial-mesenchymal transition.

Automatically generated from the 10 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Mol Cancer; Secretory RAB GTPase 3C modulates IL6-STAT3 pathway to promote colon cancer metastasis and is associated with poor prognosis.; Authors - Yu-Chan Chang
human	WB	Autophagy; RAB37-mediated autophagy guards ovarian homeostasis and function; Authors - Rongjia Zhou
mouse	WB,IP,IHC,IF	Autophagy; Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo; Authors - Shan-Ying Wu
human	WB	Nat Commun; Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane; Authors - Tong Chen ; KD Validated
human	WB	Cell Death Differ; RAB37 interacts directly with ATG5 and promotes autophagosome formation via regulating ATG5-12-16 complex assembly.; Authors - Yue Sheng
human	WB,IHC,IF	Clin Cancer Res; RAB37 Hypermethylation Regulates Metastasis and Resistance to Docetaxel-Based Induction Chemotherapy in Nasopharyngeal Carcinoma.; Authors - Yingqin Li