RAB37 Polyclonal antibody

RAB37 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 13051-1-AP

Synonyms

RAB37, Ras related protein Rab 37

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Validation Data Gallery

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  WB analysis using 13051-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13051-1-AP (RAB37 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13051-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13051-1-AP (RAB37 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13051-1-AP

  IP result of anti-RAB37 (IP:13051-1-AP, 4ug; Detection:13051-1-AP 1:3000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of mouse spleen using 13051-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13051-1-AP (RAB37 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 13051-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13051-1-AP (RAB37 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen tissue using 13051-1-AP

  IHC result from Barry Bradford of 13051-1-AP (1:1,000) in Mouse Spleen.

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

13051-1-AP targets RAB37 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB37 fusion protein Ag3717
• Full Name: RAB37, member RAS oncogene family
• Calculated Molecular Weight: 223 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC016615
• Gene Symbol: RAB37
• Gene ID (NCBI): 326624
• RRID: AB_10642695
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB37, as a small GTPase, plays important roles in several cellular processes through intracellular membrane traffic. RAB37 can regulate insulin secretion in β-cells 7 and TNF-α secretion from activated macrophages. In allergic responses, RAB37 regulated mast cell degranulation. RAB37 has been identified to be a tumor suppressor and it regulated exocytosis of several proteins including TIMP metallopeptidase inhibitor 1 (TIMP1), secreted frizzled-related protein-1 (SFRP1) 12, thrombospondin-1 (TSP1), and soluble ST2.

Publications

Species	Application	Title
human	WB,IHC,IF	Clin Cancer Res; RAB37 Hypermethylation Regulates Metastasis and Resistance to Docetaxel-Based Induction Chemotherapy in Nasopharyngeal Carcinoma.; Authors - Yingqin Li
mouse	WB,IHC,IF,IP	Autophagy; Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo; Authors - Shan-Ying Wu
human	WB	Cell Death Differ; RAB37 interacts directly with ATG5 and promotes autophagosome formation via regulating ATG5-12-16 complex assembly.; Authors - Yue Sheng
human	IHC	Mol Cancer; Secretory RAB GTPase 3C modulates IL6-STAT3 pathway to promote colon cancer metastasis and is associated with poor prognosis.; Authors - Yu-Chan Chang
canine	WB	Cell Rep; Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells.; Authors - Takahide Matsui