RAB37 Recombinant monoclonal antibody

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  WB analysis using 83740-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83740-4-RR (RAB37 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 83740-4-RR

  IP result of anti-RAB37 (IP:83740-4-RR, 4ug; Detection:83740-4-RR 1:2000) with mouse brain tissue lysate 1840 ug.

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  Affinity and Kinetic Characterization of 83740-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83740-4-RR against Human RAB37 were performed. The affinity constant is 1.25 nM.

RAB37 Uni-rAb^® Recombinant Antibody for WB, IP, ELISA

Cat No. 83740-4-RR

Clone No.240838D6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

Ras-related protein Rab-37, Ras related protein Rab 37, 240838D6

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Tested Applications

• Positive WB detected in: Jurkat cells, A549 cells, mouse brain tissues, rat brain tissues
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83740-4-RR targets RAB37 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3717

  Product name: Recombinant human RAB37 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-223 aa of BC016615

  Sequence: MTGTPGAVATRDGEAPERSPPCSPSYDLTGKVMLLGDTGVGKTCFLIQFKDGAFLSGTFIATVGIDFRNKVVTVDGVRVKLQIWDTAGQERFRSVTHAYYRDAQALLLLYDITNKSSFDNIRAWLTEIHEYAQRDVVIMLLGNKADMSSERVIRSEDGETLAREYGVPFLETSAKTGMNVELAFLAIAKELKYRAGHQADEPSFQIRDYVESQKKRSSCCSFM

• Full Name: RAB37, member RAS oncogene family
• Calculated Molecular Weight: 223 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC016615
• Gene Symbol: RAB37
• Gene ID (NCBI): 326624
• RRID: AB_3671338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96AX2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB37, as a small GTPase, plays important roles in several cellular processes through intracellular membrane traffic. RAB37 can regulate insulin secretion in β-cells 7 and TNF-α secretion from activated macrophages. In allergic responses, RAB37 regulated mast cell degranulation. RAB37 has been identified to be a tumor suppressor and it regulated exocytosis of several proteins including TIMP metallopeptidase inhibitor 1 (TIMP1), secreted frizzled-related protein-1 (SFRP1) 12, thrombospondin-1 (TSP1), and soluble ST2.