Tested Applications
Positive WB detected in | A375 cells, NIH/3T3 cells, HaCaT cells, MCF-10A cells |
Positive IHC detected in | human gliomas tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
IF | See 1 publications below |
Product Information
12234-1-AP targets RAB38 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RAB38 fusion protein Ag2874 Predict reactive species |
Full Name | RAB38, member RAS oncogene family |
Calculated Molecular Weight | 211 aa, 24 kDa |
Observed Molecular Weight | 24 kDa |
GenBank Accession Number | BC015808 |
Gene Symbol | RAB38 |
Gene ID (NCBI) | 23682 |
RRID | AB_2877839 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P57729 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAB38 (also known as rrGTPbp or NY-MEL-1) is a member of the Rab small GTPase family, which regulates intracellular vesicle trafficking and membrane transport. RAB38 is specifically expressed in lysosome-related organelle (LRO)-containing tissues and cells, such as melanocytes and platelets, where it interacts with its paralogue RAB32 to regulate melanosome biogenesis and transport melanogenic enzymes (e.g., tyrosinase, TRP1, and TRP2) from early/recycling endosomes to maturing melanosomes.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for RAB38 antibody 12234-1-AP | Download protocol |
IHC protocol for RAB38 antibody 12234-1-AP | Download protocol |
IF protocol for RAB38 antibody 12234-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
EMBO Rep Rab22A recruits BLOC-1 and BLOC-2 to promote the biogenesis of recycling endosomes. | ||
JBMR Plus Interspecies Single-Cell RNA-Seq Analysis Reveals the Novel Trajectory of Osteoclast Differentiation and Therapeutic Targets |