RAB39B Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB39B Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

11

Reviews (1)

Cat no : 12162-1-AP

Synonyms

RAB39B, Ras related protein Rab 39B

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Validation Data Gallery

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  WB analysis of fetal human brain using 12162-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12162-1-AP (RAB39B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12162-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12162-1-AP (RAB39B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12162-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12162-1-AP (RAB39B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12162-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12162-1-AP (RAB39B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12162-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12162-1-AP (RAB39B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 12162-1-AP

  IP result of anti-RAB39B (IP:12162-1-AP, 4ug; Detection:12162-1-AP 1:1000) with SH-SY5Y cells lysate 2200 ug.

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  IP experiment of mouse brain using 12162-1-AP

  IP result of anti-RAB39B (IP:12162-1-AP, 4ug; Detection:12162-1-AP 1:300) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human lymphoma using 12162-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 12162-1-AP (RAB39B antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, mouse brain tissue, Y79 cells, human brain tissue
• Positive IP detected in: SH-SY5Y cells, mouse brain tissue
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

12162-1-AP targets RAB39B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB39B fusion protein Ag2803
• Full Name: RAB39B, member RAS oncogene family
• Calculated Molecular Weight: 213 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC009714
• Gene Symbol: RAB39B
• Gene ID (NCBI): 116442
• RRID: AB_2177360
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang ; KO Validated
mouse	WB	Am J Hum Genet; Mutations in RAB39B Cause X-Linked Intellectual Disability and Early-Onset Parkinson Disease with α-Synuclein Pathology.; Authors - Gabrielle R Wilson
human	IP	EMBO J; Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.; Authors - Chantal Sellier
human	WB	Mov Disord; Clinical and Neuropathological Features Associated With Loss of RAB39B.; Authors - Yujing Gao
mouse	IF,WB	Acta Neuropathol Commun; Synaptic localization of C9orf72 regulates post-synaptic glutamate receptor 1 levels.; Authors - Shangxi Xiao
human	WB,IF	Brain Pathol; RAB39B is redistributed in dementia with Lewy bodies and is sequestered within aβ plaques and Lewy bodies.; Authors - David J Koss

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

David (Verified Customer) (09-25-2019)

The antibodiy works well in membrane enriched fractionates generated from human brain homogenate. Note that several higher molecular weight bands are seen when using crude lysates generated from the same samples.Image details: lane 1 = molecular weight ladder, lanes 2-7 = membrane enriched fractionates (2µg/lane).Membrane blocked in 5% milk powder containing TBST overnight at 4oC. Primary antibody: RAB39B (1:1000) in 5% BSA containing TBST, 1hr incubation at RT. Secondar antibody: y Goat anti Rabbit-HRP (1:5000, 1hr RT) in 5% milk powder containing TBST.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human brain tissue

RAB39B Antibody Western Blot, validation (1:1000 dilution) in Human brain tissue (Cat no:12162-1-AP)