RAB3C Polyclonal antibody

RAB3C Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10788-1-AP

Synonyms

RAB3C, Ras related protein Rab 3C



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, mouse lung tissue, rat brain tissues
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10788-1-AP targets RAB3C in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB3C fusion protein Ag1241
Full Name RAB3C, member RAS oncogene family
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC013033
Gene Symbol RAB3C
Gene ID (NCBI) 115827
RRIDAB_2177217
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab proteins are small molecular weight GTPases that control vesicular traffic in eukaryotic cells. A subset of Rab proteins, the Rab3 proteins (Rab3A, Rab3B, Rab3C, and Rab3D) are thought to play an important role in regulated exocytosis of vesicles. Although Rab3 proteins are highly homologous, displaying 77-85% amino acid identity with greatest variance evident within the N- and C-terminal regions, their subcellular targets and functional roles remain somewhat distinct between them. RT-PCR analysis indicated that human Rab3C was expressed in the human brain, placenta, and lung.

Protocols

Product Specific Protocols
WB protocol for RAB3C antibody 10788-1-APDownload protocol
IHC protocol for RAB3C antibody 10788-1-APDownload protocol
IP protocol for RAB3C antibody 10788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Proc Natl Acad Sci U S A

The GTPase Rab3b/3c-positive recycling vesicles are involved in cross-presentation in dendritic cells.

Authors - Zou Liyun L
mouseIF

PLoS Genet

Cell fate specification in the lingual epithelium is controlled by antagonistic activities of Sonic hedgehog and retinoic acid.

Authors - Maha El Shahawy
humanWB

Front Endocrinol (Lausanne)

Clinical identification of expressed proteins in adrenal medullary hyperplasia detected with hypertension

Authors - He Ma
humanIHC

Signal Transduct Target Ther

Integrative analysis of DNA methylation and gene expression identified cervical cancer-specific diagnostic biomarkers.

Authors - Wanxue Xu
humanIHC

Signal Transduct Target Ther

Integrative analysis of DNA methylation and gene expression identified cervical cancer-specific diagnostic biomarkers

Authors - Wanxue Xu