Tested Applications
| Positive WB detected in | HEK-293 cells, HL-60 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21663-1-AP targets RAB3GAP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16426 Product name: Recombinant human RAB3GAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 502-851 aa of BC146809 Sequence: PGDAGKAGDQLVPDNLKETDKEKGEVGKSWDSWSDSEEEFFECLSDTEELKGNGQESGKKGGPKEMANLRPEGRLYQHGKLTLLHNGEPLYIPVTQEPAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCSLEDFVRWYSPRDYIEEEVIDEKGNVVLKGELSARMKIPSNMWVEAWETAKPIPARRQRRLFDDTREAEKVLHYLAIQKPADLARHLLPCVIHAAVLKVKEEESLENISSVKKIIKQIISHSSKVLHFPNPEDKKLEEIIHQITNVEALIARARSLKAKFGTEKCEQEEEKEDLERFVSCLLEQPEVLVTGAGRGHAGRIIH Predict reactive species |
| Full Name | RAB3 GTPase activating protein subunit 1 (catalytic) |
| Calculated Molecular Weight | 981 aa, 111 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC146809 |
| Gene Symbol | RAB3GAP1 |
| Gene ID (NCBI) | 22930 |
| RRID | AB_10863124 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15042 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAB3GAP1 (RAB3 GTPase-activating protein 1) is the catalytic subunit of the heterodimeric enzyme RAB3GAP (RAB3GTPase-activating protein), a key regulator of the RAB3 cycle, which controls calcium-mediated exocytosis of neurotransmitters and hormones. Mutations in RAB3GAP1 cause Warburg Micro syndrome (OMIM 600118) and Martsolf syndrome (OMIM 212720), phenotypically overlapping autosomal recessive conditions characterized by ocular and neurodevelopmental manifestations.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RAB3GAP1 antibody 21663-1-AP | Download protocol |
| IHC protocol for RAB3GAP1 antibody 21663-1-AP | Download protocol |
| IP protocol for RAB3GAP1 antibody 21663-1-AP | Download protocol |
| WB protocol for RAB3GAP1 antibody 21663-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RAB3GAP1 Polyclonal antibody (Cat# 21663-1-AP) has 12 citations published in journals including Autophagy, Cell Death & Disease, EMBO Journal, J Cell Biol, J Neurosci, Neurobiology of Disease, BMC Biology, JACC Basic Translational Science, Biophysical Journal, Amino Acids, Open Biology, and Toxicology Letters. Research fields span lipophagy, colorectal cancer, cardiac exocytosis, ER structure, neurodevelopmental disorders (Warburg Micro syndrome), and synaptic vesicle fusion.
| Species | Application | Title |
|---|---|---|
Autophagy RAB18-dependent recruitment of OPTN initiates selective lipophagy in osteoblasts. | ||
Cell Death Dis A tumor-specific modulation of heterogeneous ribonucleoprotein A0 promotes excessive mitosis and growth in colorectal cancer cells.
| ||
JACC Basic Transl Sci zDHHC9 Regulates Cardiomyocyte Rab3a Activity and Atrial Natriuretic Peptide Secretion Through Palmitoylation of Rab3gap1 | ||
J Cell Biol Rab18 and a Rab18 GEF complex are required for normal ER structure.
| ||
Neurobiol Dis The Warburg micro syndrome protein RAB3GAP1 modulates neuronal morphogenesis and interacts with axon elongation end ER-Golgi trafficking factors
|
Reviews
What customers say
Both reviewers used this antibody for Western blotting and gave it a 4/5 rating. One user (HUVEC, 1:1000) confirmed detection at the correct molecular weight but noted a nonspecific band around 40 kDa. The other (mouse brain lysate, 1:3000) found it acceptable for probing RAB3GAP1 but occasionally observed a nearby nonspecific band, suggesting that 66688-1-Ig may perform better for this target. Overall, the antibody works as expected with minor background issues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sammy (Verified Customer) (11-05-2023) | Detects a band at the correct MW via western blotting. An unspecific band also detected around 40 kDa.
 |
Bing (Verified Customer) (08-04-2021) | OK to probe RAB3GAP1, but sometimes produces an unspecific band nearby. 66688-1-Ig seems better.
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