RAB3IL1 Polyclonal antibody

RAB3IL1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17827-1-AP

Synonyms

Rabin3-like 1, Rabin3 like 1, Rab3A-interacting-like protein 1, Rab-3A-interacting-like protein 1, Guanine nucleotide exchange factor for Rab-3A

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Validation Data Gallery

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  WB analysis using 17827-1-AP

  Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 17827-1-AP (RAB3IL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 17827-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17827-1-AP (RAB3IL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 17827-1-AP

  Immunofluorescent analysis of Hela cells, using RAB3IL1 antibody 17827-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, human testis tissue
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

17827-1-AP targets RAB3IL1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB3IL1 fusion protein Ag12204
• Full Name: RAB3A interacting protein (rabin3)-like 1
• Calculated Molecular Weight: 382 aa, 43 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC022239
• Gene Symbol: RAB3IL1
• Gene ID (NCBI): 5866
• RRID: AB_2300814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Small GTPases; Guanine nucleotide exchange factors activate Rab8a for Toll-like receptor signalling.; Authors - Samuel J Tong
mouse	IF	Development; Identification of a heterogeneous and dynamic ciliome during embryonic development and cell differentiation; Authors - Kelsey H Elliott