RAB5A Monoclonal antibody

RAB5A Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b


human, pig and More (1)







Cat no : 66339-1-Ig


RAB5, RAB5A, Ras related protein Rab 5A

Tested Applications

Positive WB detected inHEK-293 cells, fetal human brain tissue, pig brain tissue
Positive IHC detected inhuman liver cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66339-1-Ig targets RAB5A in WB, IF, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman, monkey
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RAB5A fusion protein Ag16781
Full Name RAB5A, member RAS oncogene family
Calculated Molecular Weight 215 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC001267
Gene Symbol RAB5A
Gene ID (NCBI) 5868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab5a belongs to the Rab GTPases family proteins, which play roles in endocytosis, exocytosis, and vesicular transportation. Rab family proteins were recently reported to play important roles in human cancer progression. Rab5a was reported to be overexpressed in breast and ovarian cancers and associated with proliferation and invasion.


Product Specific Protocols
WB protocol for RAB5A antibody 66339-1-IgDownload protocol
IHC protocol for RAB5A antibody 66339-1-IgDownload protocol
IF protocol for RAB5A antibody 66339-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Bioact Mater

Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis.

Authors - Jin Wang

Clin Ther

Differential Expression of Rab5 and Rab7 Small GTPase Proteins in Placental Tissues From Pregnancies Affected by Maternal Coronavirus Disease 2019.

Authors - Yoel Benarroch

J Exp Clin Cancer Res

TFEB-driven autophagy potentiates TGF-β induced migration in pancreatic cancer cells.

Authors - Ruizhi He

Commun Biol

Arginine GlcNAcylation of Rab small GTPases by the pathogen Salmonella Typhimurium.

Authors - Kun Meng

PLoS Pathog

ALIX and TSG101 are essential for cellular entry and replication of two porcine alphacoronaviruses

Authors - Xiongnan Chen

Cell Rep

Pten regulates endocytic trafficking of cell adhesion and Wnt signaling molecules to pattern the retina

Authors - Yacine Touahri