RAB5A Monoclonal antibody

RAB5A Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1B6A5

Cat no : 66339-1-Ig

Synonyms

RAB5, RAB5A, Ras related protein Rab 5A



Tested Applications

Positive WB detected inHEK-293 cells, fetal human brain tissue, pig brain tissue
Positive IHC detected inhuman liver cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66339-1-Ig targets RAB5A in WB, IHC, IF,ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RAB5A fusion protein Ag16781
Full Name RAB5A, member RAS oncogene family
Calculated molecular weight 215 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC001267
Gene symbol RAB5A
Gene ID (NCBI) 5868
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab5a belongs to the Rab GTPases family proteins, which play roles in endocytosis, exocytosis, and vesicular transportation. Rab family proteins were recently reported to play important roles in human cancer progression. Rab5a was reported to be overexpressed in breast and ovarian cancers and associated with proliferation and invasion.

Protocols

Product Specific Protocols
WB protocol for RAB5A antibody 66339-1-IgDownload protocol
IHC protocol for RAB5A antibody 66339-1-IgDownload protocol
IF protocol for RAB5A antibody 66339-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

TFEB-driven autophagy potentiates TGF-β induced migration in pancreatic cancer cells.

Authors - Ruizhi He

Commun Biol

Arginine GlcNAcylation of Rab small GTPases by the pathogen Salmonella Typhimurium.

Authors - Kun Meng
humanIF

Clin Ther

Differential Expression of Rab5 and Rab7 Small GTPase Proteins in Placental Tissues From Pregnancies Affected by Maternal Coronavirus Disease 2019.

Authors - Yoel Benarroch