Validation Data Gallery
|Positive WB detected in||HEK-293 cells, fetal human brain tissue, pig brain tissue|
|Positive IHC detected in||human liver cancer tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
66339-1-Ig targets RAB5A in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.
|Tested Reactivity||human, pig|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||RAB5A fusion protein Ag16781|
|Full Name||RAB5A, member RAS oncogene family|
|Calculated molecular weight||215 aa, 24 kDa|
|Observed molecular weight||24 kDa|
|GenBank accession number||BC001267|
|Gene ID (NCBI)||5868|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Rab5a belongs to the Rab GTPases family proteins, which play roles in endocytosis, exocytosis, and vesicular transportation. Rab family proteins were recently reported to play important roles in human cancer progression. Rab5a was reported to be overexpressed in breast and ovarian cancers and associated with proliferation and invasion.
Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis.
Differential Expression of Rab5 and Rab7 Small GTPase Proteins in Placental Tissues From Pregnancies Affected by Maternal Coronavirus Disease 2019.
J Exp Clin Cancer Res
TFEB-driven autophagy potentiates TGF-β induced migration in pancreatic cancer cells.
Arginine GlcNAcylation of Rab small GTPases by the pathogen Salmonella Typhimurium.