RAB5A-Specific Polyclonal antibody

RAB5A-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 20228-1-AP


RAB5, RAB5A, RAB5A-Specific, Ras related protein Rab 5A

Tested Applications

Positive WB detected inhuman brain tissue, mouse colon tissue, SH-SY5Y cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

20228-1-AP targets RAB5A-Specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name RAB5A, member RAS oncogene family
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberNM_004162
Gene Symbol RAB5A
Gene ID (NCBI) 5868
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB5A belongs to the small GTPase superfamily and Rab family. It is required for the fusion of plasma membranes and early endosomes. The antibody is specific to RAB5A. It has no cross reaction to RAB5B or RAB5C.


Product Specific Protocols
WB protocol for RAB5A-Specific antibody 20228-1-APDownload protocol
IHC protocol for RAB5A-Specific antibody 20228-1-APDownload protocol
IP protocol for RAB5A-Specific antibody 20228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Physiol

Overexpression of ameloblastin in secretory ameloblasts results in demarcated, hypomineralized opacities in enamel

Authors - Yong-Hee Patricia Chun