RAB5A-Specific Polyclonal antibody

RAB5A-Specific Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 20228-1-AP

Synonyms

RAB5, RAB5A, Ras related protein Rab 5A



Tested Applications

Positive WB detected inhuman brain tissue, mouse colon tissue, SH-SY5Y cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20228-1-AP targets RAB5A-Specific in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name RAB5A, member RAS oncogene family
Calculated molecular weight 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberNM_004162
Gene symbol RAB5A
Gene ID (NCBI) 5868
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAB5A belongs to the small GTPase superfamily and Rab family. It is required for the fusion of plasma membranes and early endosomes. The antibody is specific to RAB5A. It has no cross reaction to RAB5B or RAB5C.

Protocols

Product Specific Protocols
WB protocol for RAB5A-Specific antibody 20228-1-APDownload protocol
IHC protocol for RAB5A-Specific antibody 20228-1-APDownload protocol
IP protocol for RAB5A-Specific antibody 20228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols