Tested Applications
| Positive WB detected in | NIH/3T3 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10187-2-AP targets RAB6A in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0240 Product name: Recombinant human RAB6A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-208 aa of BC003617 Sequence: MSTGGDFGNPLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTIRLQLWDTAGQERFRSLIPSYIRDSAAAVVVYDITNVNSFQQTTKWIDDVRTERGSDVIIMLVGNKTDLADKRQVSIEEGERKAKELNVMFIETSAKAGYNVKQLFRRVAAALPGMESTQDRSREDMIDIKLEKPQEQPVSEGGCSC Predict reactive species |
| Full Name | RAB6A, member RAS oncogene family |
| Calculated Molecular Weight | 24 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC003617 |
| Gene Symbol | RAB6A |
| Gene ID (NCBI) | 5870 |
| RRID | AB_2175463 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20340 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Members of the Rab subfamily of small GTPases play an important role in the regulation of intracellular transport routes. Rab6, localized to the Golgi apparatus, has been shown to be a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). The expression of Rab6 is ubiquitous.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RAB6A antibody 10187-2-AP | Download protocol |
| IP protocol for RAB6A antibody 10187-2-AP | Download protocol |
| WB protocol for RAB6A antibody 10187-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RAB6A Polyclonal antibody (Cat# 10187-2-AP) has 15 citations across journals including Nature Communications, Journal of Clinical Investigation, Nature Structural & Molecular Biology, EMBO Journal, Journal of Cell Biology, International Journal of Molecular Sciences, Journal of Lipid Research, Development, Veterinary Research, Journal of Neuroimmunology, Neuroscience Letters, and Biology Open. Research fields span vesicle trafficking, viral replication, cancer secretory pathways, ALS/neurodegeneration, Golgi biogenesis, lipid metabolism, axon guidance, autoimmune cerebellar ataxia, and photoreceptor biology.
| Species | Application | Title |
|---|---|---|
Nat Commun Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane
| ||
Nat Commun SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors. | ||
J Clin Invest A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma. | ||
Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. | ||
J Cell Biol Sec23IP recruits VPS13B/COH1 to ER exit site-Golgi interface for tubular ERGIC formation
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Reviews
What customers say
The single review rates the product 5 stars, praising its performance in both Western Blot and Immunofluorescence. For WB, it produced a strong, specific band at the expected molecular weight. For IF using PFA fixation, it successfully labeled Rab6 at the Golgi and cilia. Overall, the reviewer found the antibody to be very good and reliable across both applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (01-25-2019) | Very good, both for WB and IF assays.By WB, it gives a strong specific band at the expected molecular weight; by IF, PFA fixation, it labels Rab6 at the Golgi and the cilia.
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