|Positive WB detected in||mouse brain tissue, NIH/3T3 cells, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10187-2-AP targets RAB6A in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RAB6A fusion protein Ag0240|
|Full Name||RAB6A, member RAS oncogene family|
|Calculated molecular weight||24 kDa|
|Observed molecular weight||24 kDa|
|GenBank accession number||BC003617|
|Gene ID (NCBI)||5870|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Members of the Rab subfamily of small GTPases play an important role in the regulation of intracellular transport routes. Rab6, localized to the Golgi apparatus, has been shown to be a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). The expression of Rab6 is ubiquitous.
A crucial role for Arf6 in the response of commissural axons to Slit.
SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Nat Struct Mol Biol
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A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (01-25-2019)
Very good, both for WB and IF assays.By WB, it gives a strong specific band at the expected molecular weight; by IF, PFA fixation, it labels Rab6 at the Golgi and the cilia.