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  • KD/KO Validated

RAB6A Polyclonal antibody

RAB6A Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 10187-2-AP

Synonyms

Rab 6, RAB6, RAB6A, RAB6A', RAB6B, Ras related protein Rab 6A



Tested Applications

Positive WB detected inmouse brain tissue, NIH/3T3 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10187-2-AP targets RAB6A in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB6A fusion protein Ag0240
Full Name RAB6A, member RAS oncogene family
Calculated molecular weight 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC003617
Gene symbol RAB6A
Gene ID (NCBI) 5870
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Members of the Rab subfamily of small GTPases play an important role in the regulation of intracellular transport routes. Rab6, localized to the Golgi apparatus, has been shown to be a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). The expression of Rab6 is ubiquitous.

Protocols

Product Specific Protocols
WB protocol for RAB6A antibody 10187-2-APDownload protocol
IF protocol for RAB6A antibody 10187-2-APDownload protocol
IP protocol for RAB6A antibody 10187-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Development

A crucial role for Arf6 in the response of commissural axons to Slit.

Authors - Mariko Kinoshita-Kawada
  • KD Validated
humanWB

Nat Commun

SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.

Authors - Seiichi Koike
mouseWB

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo
humanWB

J Clin Invest

A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma.

Authors - Xiaochao Tan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (01-25-2019)

Very good, both for WB and IF assays.By WB, it gives a strong specific band at the expected molecular weight; by IF, PFA fixation, it labels Rab6 at the Golgi and the cilia.

  • Applications: Western Blot,Immunofluorescence,