RAB8A Polyclonal antibody

RAB8A Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, canine, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 30636-1-AP

Synonyms

MEL, Oncogene c mel, RAB8, RAB8A, Ras related protein Rab 8A

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Validation Data Gallery

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  WB analysis using 30636-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30636-1-AP (RAB8A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of MDCK using 30636-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using RAB8A antibody (30636-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of NIH/3T3 using 30636-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using RAB8A antibody (30636-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, HepG2 cells
• Positive IF detected in: NIH/3T3 cells, MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30636-1-AP targets RAB8A in WB, IF, ELISA applications and shows reactivity with Human, canine, mouse samples.

• Tested Reactivity: Human, canine, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB8A fusion protein Ag30567
• Full Name: RAB8A, member RAS oncogene family
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: NM_005370
• Gene Symbol: RAB8A
• Gene ID (NCBI): 4218
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring. RAB8 has two isoforms, RAB8A and RAB8B, that share 40% amino acid identity in the C-terminal variable region. RAB8 activity is regulated by specific guanine nucleotide exchange factors (GEFs), that mediate GTP loading, and GTPase activating proteins (GAPs) that convert them into the inactive GDP-bound form. RAB8 participates in recycling of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the dendritic spine surface as well as in the intracellular transport of the metabotropic glutamate receptor type 1 in neuronal cells. Rab8 is activated at the base of the cilium by its guanine nucleotide exchanger Rabin8. The antibody 11792-1-AP can detect both RAB8A and RAB8B.