RAB8A Polyclonal antibody

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  WB analysis using 55296-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 55296-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 55296-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 55296-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 55296-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 55296-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55296-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 55296-1-AP

  IP result of anti-RAB8A (IP:55296-1-AP, 4ug; Detection:55296-1-AP 1:600) with HeLa cells lysate 1320 ug.

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  IHC staining of human colon cancer using 55296-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55296-1-AP (RAB8A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 55296-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55296-1-AP (RAB8A Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of NIH/3T3 using 55296-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RAB8A antibody (55296-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

RAB8A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 55296-1-AP

Publications(32)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

MEL, Oncogene c mel, RAB8, RAB8A, Ras related protein Rab 8A

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Tested Applications

• Positive WB detected in: A431 cells, mouse lung tissue, HepG2 cells, NIH/3T3 cells, human brain tissue, mouse skin tissue, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 14 publications below
• IP: See 2 publications below
• RIP: See 1 publications below

Product Information

55296-1-AP targets RAB8A in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  fusion protein

• Full Name: RAB8A, member RAS oncogene family
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: NM_005370
• Gene Symbol: RAB8A
• Gene ID (NCBI): 4218
• RRID: AB_10858398
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P61006
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring. RAB8 has two isoforms, RAB8A and RAB8B, that share 40% amino acid identity in the C-terminal variable region. RAB8 activity is regulated by specific guanine nucleotide exchange factors (GEFs), that mediate GTP loading, and GTPase activating proteins (GAPs) that convert them into the inactive GDP-bound form. RAB8 participates in recycling of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the dendritic spine surface as well as in the intracellular transport of the metabotropic glutamate receptor type 1 in neuronal cells. Rab8 is activated at the base of the cilium by its guanine nucleotide exchanger Rabin8. The antibody 11792-1-AP can detect both RAB8A and RAB8B. 55295-1-AP is specific to RAB8B and 55296-1-AP is specific to RAB8A.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
canine	WB,IF	Nat Cell Biol; A molecular network for de novo generation of the apical surface and lumen.; Authors - Bryant David M DM ; KD Validated
mouse	WB,IF	Cell Res; NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.; Authors - Min Liu
human	RIP	Adv Sci (Weinh); Primary Cilia Formation Mediated by Hsa_Circ_0005185/OTUB1/RAB8A Complex Inhibits Prostate Cancer Progression by Suppressing Hedgehog Signaling Pathway; Authors - Aoyu Fan
mouse	IF	Circ Res; Rasip1-Mediated Rho GTPase Signaling Regulates Blood Vessel Tubulogenesis via Nonmuscle Myosin II.; Authors - David M Barry
human	WB, IF	J Cell Biol; Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.; Authors - Michael C Burke
mouse	IF	Nat Commun; Ciliopathy-associated gene Cc2d2a promotes assembly of subdistal appendages on the mother centriole during cilia biogenesis.; Authors - Shobi Veleri

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sara (Verified Customer) (04-30-2020)

Unfortunately this antibody did not work for my samples. I followed all the instructions in the "WB protocol for Rab8a antibody 55296-1-AP" that Proteintech provides. I did not get almost any signal when following those instructions (primary antibody 1:600 for 1h at 30-35°C). After repeating the experiment incubating the membrane with 1:600 primary antibody O/N at 4°C I got better signal, but still couldn't see a band of 24 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:600
• Cell Tissue Type: Primary B cells from mouse spleen