Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11420-1-AP targets RAB9A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1969 Product name: Recombinant human RAB9A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-201 aa of BC017265 Sequence: MAGKSSLFKVILLGDGGVGKSSLMNRYVTNKFDTQLFHTIGVEFLNKDLEVDGHFVTMQIWDTAGQERFRSLRTPFYRGSDCCLLTFSVDDSQSFQNLSNWKKEFIYYADVKEPESFPFVILGNKIDISERQVSTEEAQAWCRDNGDYPYFETSAKDATNVAAAFEEAVRRVLATEDRSDHLIQTDTVNLHRKPKPSSSCC Predict reactive species |
| Full Name | RAB9A, member RAS oncogene family |
| Calculated Molecular Weight | 201 aa, 23 kDa |
| Observed Molecular Weight | 23 kDa |
| GenBank Accession Number | BC017265 |
| Gene Symbol | RAB9A |
| Gene ID (NCBI) | 9367 |
| RRID | AB_2175327 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51151 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAB9A belongs to the small GTPase superfamily, the members of which play an important role in regulating vesicular trafficking and reside in specific intracellular compartments. RAB9A has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors, such as mannose 6-phosphate receptor, from early endosomes to the trans Golgi network.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RAB9A antibody 11420-1-AP | Download protocol |
| IHC protocol for RAB9A antibody 11420-1-AP | Download protocol |
| WB protocol for RAB9A antibody 11420-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RAB9A Polyclonal antibody (Cat# 11420-1-AP) has 10 citations published in journals including Nature Neuroscience, Nucleic Acids Research, Science Signaling, International Journal of Molecular Sciences, Molecular Diversity, Journal of Virology, Journal of General Virology, Technology in Cancer Research & Treatment, Virology, and bioRxiv. Associated research fields span Alzheimer's disease, mitophagy, Wnt signaling in hematopoietic stem cells, ALS, lung cancer PROTAC design, coronavirus entry, prion biology, and gastric cancer.
| Species | Application | Title |
|---|---|---|
Nat Neurosci Mitochondrial accumulation and lysosomal dysfunction result in mitochondrial plaques in Alzheimer's disease | ||
Sci Signal EGFR-dependent endocytosis of Wnt9a and Fzd9b promotes β-catenin signaling during hematopoietic stem cell development in zebrafish | ||
Int J Mol Sci The Amyotrophic Lateral Sclerosis M114T PFN1 Mutation Deregulates Alternative Autophagy Pathways and Mitochondrial Homeostasis. | ||
Mol Divers Discovery of the novel celastrol-based PROTACs for the treatment of non-small cell lung cancer | ||
J Virol Porcine Enteric Alphacoronavirus Entry through Multiple Pathways (Caveolae, Clathrin, and Macropinocytosis) Requires Rab GTPases for Endosomal Transport |
