RABEP1 Polyclonal antibody

RABEP1 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF

Conjugate

Unconjugated

Cat no : 14350-1-AP

Synonyms

RAB5EP, Rabaptin 4, Rabaptin 5, Rabaptin 5alpha, RABEP1, RABPT5, RABPT5A



Tested Applications

Positive WB detected inHeLa cells, A431 cells, human liver tissue
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14350-1-AP targets RABEP1 in WB, IP, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RABEP1 fusion protein Ag5693
Full Name rabaptin, RAB GTPase binding effector protein 1
Calculated molecular weight 99 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC041700
Gene symbol RABEP1
Gene ID (NCBI) 9135
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RABEP1 antibody 14350-1-APDownload protocol
IF protocol for RABEP1 antibody 14350-1-APDownload protocol
IP protocol for RABEP1 antibody 14350-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Mol Cell Cardiol

Decreased inspired oxygen stimulates de novo formation of coronary collaterals in adult heart.

Authors - Amir Aghajanian
humanWB

J Cell Sci

His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation

Authors - Gabrielle Parkinson
  • KD Validated
humanIF,IP

Elife

Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.

Authors - Dawei Wang