RABEP1 Polyclonal antibody

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  WB analysis of HeLa using 14350-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14350-1-AP (RABEP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 14350-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14350-1-AP (RABEP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14350-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14350-1-AP (RABEP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14350-1-AP

  IP result of anti-RABEP1 (IP:14350-1-AP, 3ug; Detection:14350-1-AP 1:500) with HeLa cells lysate 3800ug.

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  IF Staining of HeLa using 14350-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14350-1-AP (RABEP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

RABEP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 14350-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Rabaptin-4, Rabaptin 5alpha, Rabaptin 5, Rabaptin 4, RAB5EP

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, human liver tissue
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

14350-1-AP targets RABEP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5693

  Product name: Recombinant human RABEP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 561-862 aa of BC041700

  Sequence: QLMLRQANDQLEKTMKDKQELEDFIKQSSEDSSHQISALVLRAQASEILLEELQQGLSQAKRDVQEQMAVLMQSREQVSEELVRLQKDNDSLQGKHSLHVSLQQAEDFILPDTTEALRELVLKYREDIINVRTAADHVEEKLKAEILFLKEQIQAEQCLKENLEETLQLEIENCKEEIASISSLKAELERIKVEKGQLESTLREKSQQLESLQEIKISLEEQLKKETAAKATVEQLMFEEKNKAQRLQTELDVSEQVQRDFVKLSQTLQVQLERIRQADSLERIRAILNDTKLTDINQLPET

• Full Name: rabaptin, RAB GTPase binding effector protein 1
• Calculated Molecular Weight: 99 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC041700
• Gene Symbol: RABEP1
• Gene ID (NCBI): 9135
• RRID: AB_2175644
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15276
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab GTPase-binding effector protein 1 is an enzyme that in humans is encoded by the RABEP1 gene. It belongs to rabaptin protein family. RABEP1 act as linker between gamma-adaptin, RAB4A and RAB5A. It is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. (PMID: 22841712)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF,IP	Elife; Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.; Authors - Dawei Wang
Mouse		Stroke; Variants of Rab GTPase-Effector Binding Protein-2 Cause Variation in the Collateral Circulation and Severity of Stroke.; Authors - Jennifer L Lucitti
human	WB	J Cell Sci; His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation; Authors - Gabrielle Parkinson ; KD Validated
mouse	WB	J Mol Cell Cardiol; Decreased inspired oxygen stimulates de novo formation of coronary collaterals in adult heart.; Authors - Amir Aghajanian
zebrafish	WB	FASEB J; ATP7A Maintains Bactericidal Function of Neutrophils and Macrophages via Regulating the Formation and Activation of Phagolysosomes; Authors - YuanYuan Jing