Tested Applications
| Positive WB detected in | HepG2 cells, mouse liver tissue, Jurkat cells, L02 cells, PC-3 cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human cervical cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14625-1-AP targets RABEP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6197 Product name: Recombinant human RABEP2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-299 aa of BC058900 Sequence: MAAAAPVAADDDERRRRPGAALEDSRSQEGANGEAESGELSRLRAELAGALAEMETMKAVAEVSESTKAEAVAAVQRQCQEEVASLQAILKDSISSYEAQITALKQERQQQQQDCEEKERELGRLKQLLSRAYPLDSLEKQMEKAHEDSEKLREIVLPMEKEIEELKAKLLRAEELIQEIQRRPRHAPSLHGSTELLPLSRDPSPPLEPLEELSGDGGPAAEAFAHNCDDSASISSFSLGGGVGSSSSLPQSRQGLSPEQEETASLVSTGTLVPEGIYLPPPGYQLVPDTQWEQLQTEM Predict reactive species |
| Full Name | rabaptin, RAB GTPase binding effector protein 2 |
| Calculated Molecular Weight | 64 kDa |
| Observed Molecular Weight | 59-62 kDa |
| GenBank Accession Number | BC058900 |
| Gene Symbol | RABEP2 |
| Gene ID (NCBI) | 79874 |
| RRID | AB_2175655 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H5N1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RABEP2, also named as RABPT5B, plays a role in membrane trafficking and in homotypic early endosome fusion. It has two isoforms with MW 59-63 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RABEP2 antibody 14625-1-AP | Download protocol |
| IHC protocol for RABEP2 antibody 14625-1-AP | Download protocol |
| IP protocol for RABEP2 antibody 14625-1-AP | Download protocol |
| WB protocol for RABEP2 antibody 14625-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RABEP2 Polyclonal Antibody (Cat# 14625-1-AP) has 7 citations published in J Hematol Oncol, Stroke, Arterioscler Thromb Vasc Biol, Am J Hum Genet, Biomolecules, J Mol Cell Cardiol, and Sci Rep. Research fields span cholangiocarcinoma, ischemic stroke and cerebral collateral circulation, bone marrow mesenchymal stem cells, pancreatic cancer metastasis, coronary collateral formation, and GSK3-mediated signaling. Applications cited include WB and IF in human and mouse samples.
| Species | Application | Title |
|---|---|---|
J Hematol Oncol LncRNAs H19 and HULC, activated by oxidative stress, promote cell migration and invasion in cholangiocarcinoma through a ceRNA manner. | ||
Stroke Variants of Rab GTPase-Effector Binding Protein-2 Cause Variation in the Collateral Circulation and Severity of Stroke. | ||
Arterioscler Thromb Vasc Biol Hypoxia-Preconditioned Bone Marrow Mesenchymal Stem Cells Improved Cerebral Collateral Circulation and Stroke Outcome in Mice | ||
Am J Hum Genet A cross-species approach using an in vivo evaluation platform in mice demonstrates that sequence variation in human RABEP2 modulates ischemic stroke outcomes | ||
Biomolecules 5'-tRF-19-Q1Q89PJZ Suppresses the Proliferation and Metastasis of Pancreatic Cancer Cells via Regulating Hexokinase 1-Mediated Glycolysis | ||
Sci Rep Rab-GTPase binding effector protein 2 (RABEP2) is a primed substrate for Glycogen Synthase kinase-3 (GSK3). |
Reviews
What customers say
The single review for this product is a 5-star rating. The reviewer describes it as an excellent antibody for detecting RABEP2 by both Western Blot and Immunofluorescence. It was tested on HUVEC cells using a 1:1000 dilution for WB and 1:200 for IF, with positive results in both applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sammy (Verified Customer) (04-22-2024) | Excellent antibody for detection of RABEP2 by WB or IF.
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