RABEP2 Polyclonal antibody

RABEP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14625-1-AP

Synonyms

FRA, Rabaptin 5beta, RABEP2, RABPT5B



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman cervical cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14625-1-AP targets RABEP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RABEP2 fusion protein Ag6197
Full Name rabaptin, RAB GTPase binding effector protein 2
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 59-62 kDa
GenBank Accession NumberBC058900
Gene Symbol RABEP2
Gene ID (NCBI) 79874
RRIDAB_2175655
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RABEP2, also named as RABPT5B, plays a role in membrane trafficking and in homotypic early endosome fusion. It has two isoforms with MW 59-63 kDa.

Protocols

Product Specific Protocols
WB protocol for RABEP2 antibody 14625-1-APDownload protocol
IHC protocol for RABEP2 antibody 14625-1-APDownload protocol
IF protocol for RABEP2 antibody 14625-1-APDownload protocol
IP protocol for RABEP2 antibody 14625-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Hum Genet

A cross-species approach using an in vivo evaluation platform in mice demonstrates that sequence variation in human RABEP2 modulates ischemic stroke outcomes

Authors - Han Kyu Lee
mouseWB

Stroke

Variants of Rab GTPase-Effector Binding Protein-2 Cause Variation in the Collateral Circulation and Severity of Stroke.

Authors - Jennifer L Lucitti
humanWB,IF

Sci Rep

Rab-GTPase binding effector protein 2 (RABEP2) is a primed substrate for Glycogen Synthase kinase-3 (GSK3).

Authors - Lisa Logie
mouseWB

J Mol Cell Cardiol

Decreased inspired oxygen stimulates de novo formation of coronary collaterals in adult heart.

Authors - Amir Aghajanian
mouseWB

J Hematol Oncol

LncRNAs H19 and HULC, activated by oxidative stress, promote cell migration and invasion in cholangiocarcinoma through a ceRNA manner.

Authors - Wen-Tao Wang
mouseWB,IF

Arterioscler Thromb Vasc Biol

Hypoxia-Preconditioned Bone Marrow Mesenchymal Stem Cells Improved Cerebral Collateral Circulation and Stroke Outcome in Mice

Authors - Hao Tian