RABEPK/p40 Polyclonal antibody

RABEPK/p40 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 15105-1-AP

Synonyms

40 kDa Rab9 effector protein, p40, RAB9P40, RABEPK



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15105-1-AP targets RABEPK/p40 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RABEPK/p40 fusion protein Ag2593
Full Name Rab9 effector protein with kelch motifs
Calculated molecular weight 41 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC065725
Gene symbol RABEPK
Gene ID (NCBI) 10244
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.

Protocols

Product Specific Protocols
WB protocol for RABEPK/p40 antibody 15105-1-APDownload protocol
IHC protocol for RABEPK/p40 antibody 15105-1-APDownload protocol
IF protocol for RABEPK/p40 antibody 15105-1-APDownload protocol
IP protocol for RABEPK/p40 antibody 15105-1-APDownload protocol
FC protocol for RABEPK/p40 antibody 15105-1-APDownload protocol
Standard Protocols
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