• Featured Product
  • KD/KO Validated

RABGGTA Polyclonal antibody

RABGGTA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14448-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

EC:2.5.1.60, Geranylgeranyl transferase type-2 subunit alpha, Rab geranyl-geranyltransferase subunit alpha, Rab GG transferase alpha, Rab GGTase alpha

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IHC detected inhuman lung tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14448-1-AP targets RABGGTA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RABGGTA fusion protein Ag5923 Predict reactive species
Full Name Rab geranylgeranyltransferase, alpha subunit
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 60~65 kDa
GenBank Accession NumberBC003093
Gene Symbol RABGGTA
Gene ID (NCBI) 5875
RRIDAB_10644135
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92696
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GGTase II (RAB geranylgeranyl transferase, RABGGTase) is a heterodimer composed of tightly bound α- and β-subunits, which belongs to the Ras superfamily of small monomeric GTPases. RABGGTase is extensively involved in intracellular membrane trafficking of membrane-bound organelles and vesicles and specifically catalyses the covalent attachment of GGPP to the cysteine of a carboxyl-terminal CC or CXC motif of RAB proteins (PMID 11136552, 10737774, 35961942).

Protocols

Product Specific Protocols
WB protocol for RABGGTA antibody 14448-1-APDownload protocol
IHC protocol for RABGGTA antibody 14448-1-APDownload protocol
IF protocol for RABGGTA antibody 14448-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Pathog

Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival.

Authors - Liang Wei Wang
  • KD Validated
mouseWB

Cell Death Dis

Simvastatin accelerated motoneurons death in SOD1G93A mice through inhibiting Rab7-mediated maturation of late autophagic vacuoles.

Authors - Lin Bai
mouseWB,IF

Brain Res Bull

RABGGTB plays a critical role in ALS pathogenesis

Authors - Haiyang Ma
Loading...