RABGGTA Polyclonal antibody

• Featured Product
• KD/KO Validated

RABGGTA Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 14448-1-AP

Synonyms

PTAR3, Rab GG transferase alpha, Rab GGTase alpha, RABGGTA

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Validation Data Gallery

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  WB analysis of HEK-293 using 14448-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14448-1-AP (RABGGTA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14448-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14448-1-AP (RABGGTA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 14448-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14448-1-AP (RABGGTA antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 14448-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14448-1-AP (RABGGTA antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 14448-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14448-1-AP (RABGGTA antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 14448-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14448-1-AP (RABGGTA antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 14448-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14448-1-AP (RABGGTA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells
• Positive IHC detected in: human lung tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

14448-1-AP targets RABGGTA in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RABGGTA fusion protein Ag5923
• Full Name: Rab geranylgeranyltransferase, alpha subunit
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC003093
• Gene Symbol: RABGGTA
• Gene ID (NCBI): 5875
• RRID: AB_10644135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GGTase II (RAB geranylgeranyl transferase, RABGGTase) is a heterodimer composed of tightly bound α- and β-subunits, which belongs to the Ras superfamily of small monomeric GTPases. RABGGTase is extensively involved in intracellular membrane trafficking of membrane-bound organelles and vesicles and specifically catalyses the covalent attachment of GGPP to the cysteine of a carboxyl-terminal CC or CXC motif of RAB proteins (PMID 11136552, 10737774, 35961942).

Publications

Species	Application	Title
human	WB	PLoS Pathog; Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival.; Authors - Liang Wei Wang ; KD Validated
mouse	WB	Cell Death Dis; Simvastatin accelerated motoneurons death in SOD1G93A mice through inhibiting Rab7-mediated maturation of late autophagic vacuoles.; Authors - Lin Bai
mouse	WB,IF	Brain Res Bull; RABGGTB plays a critical role in ALS pathogenesis; Authors - Haiyang Ma