IFT22 Polyclonal antibody

IFT22 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26896-1-AP

Synonyms

IFT22, Rab like protein 5, RABL5

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Validation Data Gallery

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  WB analysis of HeLa using 26896-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26896-1-AP (IFT22 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 26896-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26896-1-AP (IFT22 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 26896-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26896-1-AP (IFT22 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 26896-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26896-1-AP (IFT22 antibody at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26896-1-AP is IFT22 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFT22 fusion protein Ag25292
• Full Name: RAB, member RAS oncogene family-like 5
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC038668
• Gene Symbol: RABL5
• Gene ID (NCBI): 64792
• RRID: AB_2880672
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.