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RAC2 Polyclonal antibody

RAC2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10735-1-AP

Synonyms

EN 7, Gx, HSPC022, p21 Rac2, RAC2, Small G protein



Tested Applications

Positive WB detected inA431 cells
Positive IP detected inHL-60 cells
Positive IHC detected inhuman tonsillitis tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10735-1-AP targets RAC2 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAC2 fusion protein Ag1202
Full Name ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC001485
Gene symbol RAC2
Gene ID (NCBI) 5880
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAC2, also named as GX, belongs to the small GTPase superfamily and Rho family. RAC2 is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. RAC2 augments the production of reactive oxygen species (ROS) by NADPH oxidase. Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome.

Protocols

Product Specific Protocols
WB protocol for RAC2 antibody 10735-1-APDownload protocol
IHC protocol for RAC2 antibody 10735-1-APDownload protocol
IP protocol for RAC2 antibody 10735-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Tumour Biol

AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis.

Authors - Zhaoyang Zeng
humanWB

J Exp Clin Cancer Res

Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway.

Authors - Yu Lian
mouseWB

Nat Genet

In vivo RNAi screening identifies regulators of actin dynamics as key determinants of lymphoma progression.

Authors - Meacham Corbin E CE
  • KD Validated