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  • KD/KO Validated

RAC2 Polyclonal antibody

RAC2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 10735-1-AP


EN 7, Gx, HSPC022, p21 Rac2, RAC2, Small G protein

Tested Applications

Positive WB detected inA431 cells, HL-60 cells, mouse spleen tissue, rat spleen tissue
Positive IP detected inHL-60 cells
Positive IHC detected inhuman tonsillitis tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10735-1-AP targets RAC2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAC2 fusion protein Ag1202
Full Name ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC001485
Gene Symbol RAC2
Gene ID (NCBI) 5880
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAC2, also named as GX, belongs to the small GTPase superfamily and Rho family. RAC2 is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. RAC2 augments the production of reactive oxygen species (ROS) by NADPH oxidase. Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome.


Product Specific Protocols
WB protocol for RAC2 antibody 10735-1-APDownload protocol
IHC protocol for RAC2 antibody 10735-1-APDownload protocol
IP protocol for RAC2 antibody 10735-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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