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  • KD/KO Validated

RAC2 Monoclonal antibody

RAC2 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, coIP,ELISA

Conjugate

Unconjugated

CloneNo.

4C2H6

Cat no : 60077-1-Ig

Synonyms

EN 7, Gx, HSPC022, p21 Rac2, RAC2, Small G protein



Tested Applications

Positive WB detected inJurkat cells, RAW264.7 cells, NIH/3T3 cells, HSC-T6 cells, MCF-7 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Sample-dependent, check data in validation data gallery

Product Information

60077-1-Ig targets RAC2 in WB, IHC, coIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAC2 fusion protein Ag1202
Full Name ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC001485
Gene symbol RAC2
Gene ID (NCBI) 5880
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAC2, also named as GX, belongs to the small GTPase superfamily and Rho family. RAC2 is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. RAC2 augments the production of reactive oxygen species (ROS) by NADPH oxidase. Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome.

Protocols

Product Specific Protocols
WB protocol for RAC2 antibody 60077-1-IgDownload protocol
IHC protocol for RAC2 antibody 60077-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Yakugaku Zasshi

Induction of differentiation by panaxydol in human hepatocarcinoma SMMC-7721 cells via cAMP and MAP kinase dependent mechanism.

Authors - Wang Ze-Jian ZJ
humanWB,IHC

Cancer Med

Nonconserved miR-608 suppresses prostate cancer progression through RAC2/PAK4/LIMK1 and BCL2L1/caspase-3 pathways by targeting the 3'-UTRs of RAC2/BCL2L1 and the coding region of PAK4.

Authors - Xu Zhang
  • KD Validated
humanWB,IHC

Int J Oncol

RAC2 acts as a prognostic biomarker and promotes the progression of clear cell renal cell carcinoma.

Authors - Yuenan Liu
  • KD Validated
humanWB

J Biol Chem

The actin regulatory protein HS1 interacts with Arp2/3 and mediates efficient neutrophil chemotaxis.

Authors - Cavnar Peter J PJ
humanWB

Oncotarget

Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma.

Authors - Hao Bo
mouseWB

Sci Signal

Rac signal adaptation controls neutrophil mobilization from the bone marrow.

Authors - Carlo Cosimo Campa