RAC1/2/3 Polyclonal antibody

• Featured Product
• KD/KO Validated

RAC1/2/3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 16117-1-AP

Synonyms

RAC3, Ras-related C3 botulinum toxin substrate 3, p21-Rac3, p21 Rac3, EC:3.6.5.2

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Validation Data Gallery

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  WB analysis using 16117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16117-1-AP (RAC1/2/3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16117-1-AP (RAC1/2/3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16117-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16117-1-AP (RAC1/2/3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16117-1-AP

  IP result of anti-RAC1/2/3 (IP:16117-1-AP, 3ug; Detection:16117-1-AP 1:500) with mouse brain tissue lysate 2640ug.

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  IHC staining of mouse brain using 16117-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16117-1-AP (RAC1/2/3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16117-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16117-1-AP (RAC1/2/3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, human brain tissue, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IP: See 1 publications below

Product Information

16117-1-AP targets RAC1/2/3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAC1/2/3 fusion protein Ag9045
• Full Name: ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3)
• Calculated Molecular Weight: 192 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC015197
• Gene Symbol: RAC3
• Gene ID (NCBI): 5881
• RRID: AB_2176154
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rac3 is a GTPase that belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. There are three Rac genes in mammals, which share over 90% protein identity but differ for their pattern of expression. Rac1 is ubiquitous, Rac2 is specific for hematopoietic cells, while Rac3 is prevalent in the developing nervous system. This antibody can recognize Rac1, Rac2, and Rac3.

Publications

Species	Application	Title
human	WB	Mol Cancer; F-box protein complex FBXL19 regulates TGFβ1-induced E-cadherin down-regulation by mediating Rac3 ubiquitination and degradation.; Authors - Su Dong
mouse	WB	Front Cell Neurosci; A Method to Culture GABAergic Interneurons Derived from the Medial Ganglionic Eminence.; Authors - Sira A Franchi ; KO Validated
human	WB	Mol Biol Cell; Phosphorylated cortactin recruits Vav2 guanine nucleotide exchange factor to activate Rac3 and promote invadopodial function in invasive breast cancer cells.; Authors - Brian J Rosenberg ; KD Validated
human	WB	BMC Cancer; Rac3 induces a molecular pathway triggering breast cancer cell aggressiveness: differences in MDA-MB-231 and MCF-7 breast cancer cell lines.; Authors - Gest Caroline C
mouse	WB	Glycoconj J; Gangliosides in the differentiation process of primary neurons: the specific role of GM1-oligosaccharide.; Authors - Erika Di Biase
human	WB	Asian Pac J Cancer Prev; Silencing of rac3 inhibits proliferation and induces apoptosis of human lung cancer cells.; Authors - Tie-Qin Liu ; KD Validated