Tested Applications
| Positive WB detected in | K-562 cells, A431 cells, HeLa cells, mouse testis tissue, RAW 264.7 cells |
| Positive IP detected in | mouse testis tissue, HeLa cells, K-562 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13739-1-AP targets RACGAP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4676 Product name: Recombinant human RACGAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 334-632 aa of BC032754 Sequence: PCIPTLIGTPVKIGEGMLADFVSQTSPMIPSIVVHCVNEIEQRGLTETGLYRISGCDRTVKELKEKFLRVKTVPLLSKVDDIHAICSLLKDFLRNLKEPLLTFRLNRAFMEAAEITDEDNSIAAMYQAVGELPQANRDTLAFLMIHLQRVAQSPHTKMDVANLAKVFGPTIVAHAVPNPDPVTMLQDIKRQPKVVERLLSLPLEYWSQFMMVEQENIDPLHVIENSNAFSTPQTPDIKVSLLGPVTTPEHQLLKTPSSSSLSQRVRSTLTKNTPRFGSKSKSATNLGRQGNFFASPMLK Predict reactive species |
| Full Name | Rac GTPase activating protein 1 |
| Calculated Molecular Weight | 632 aa, 71 kDa |
| Observed Molecular Weight | 71 kDa |
| GenBank Accession Number | BC032754 |
| Gene Symbol | RACGAP1 |
| Gene ID (NCBI) | 29127 |
| RRID | AB_2176276 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H0H5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RACGAP1 antibody 13739-1-AP | Download protocol |
| IP protocol for RACGAP1 antibody 13739-1-AP | Download protocol |
| WB protocol for RACGAP1 antibody 13739-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RACGAP1 antibody (Cat# 13739-1-AP) has 13 citations. Journals include Cell Death & Disease, Apoptosis, International Immunopharmacology, American Journal of Physiology, Frontiers in Pharmacology, Frontiers in Cell and Developmental Biology, Translational Oncology, Scientific Reports, Cancers, BBA Molecular Cell Research, Molecular Biology of the Cell, and Journal of the Egyptian National Cancer Institute. Research fields span cancer biology (HCC, bladder, lung, renal, glioma, breast, nasopharyngeal), RACGAP1/RhoA/ERK signaling, diabetic nephropathy, aortic valve calcification, and cell cycle/meiosis.
| Species | Application | Title |
|---|---|---|
Cell Death Dis PLAGL2 promotes bladder cancer progression via RACGAP1/RhoA GTPase/YAP1 signaling | ||
Cell Death Dis Pseudogene RACGAP1P activates RACGAP1/Rho/ERK signalling axis as a competing endogenous RNA to promote hepatocellular carcinoma early recurrence. | ||
Apoptosis Oncogenic and immunological roles of RACGAP1 in pan-cancer and its potential value in nasopharyngeal carcinoma | ||
Int Immunopharmacol RACGAP1 is a pivotal gene in lung adenocarcinoma-associated membranous nephropathy: Based on comprehensive bioinformatics analysis and machine learning | ||
Am J Physiol Cell Physiol Biological and metabolomic insights into RACGAP1-mediated growth and progression of clear cell renal cell carcinoma
| ||
Front Pharmacol Effects of Shenkang Pills on Early-Stage Diabetic Nephropathy in db/db Mice via Inhibiting AURKB/RacGAP1/RhoA Signaling Pathway. |
Reviews
What customers say
The single review for 13739-1-AP is a 5-star rating. The user tested the antibody on U2OS cells at a 1:10,000 dilution for Western Blot and Immunoprecipitation. They reported that it worked very well for immunoprecipitating RACGAP1 (and KIF23) from cells arrested in G2/M phase, and also successfully detected the protein in extracts. Overall, the feedback is highly positive, indicating strong performance in both IP and WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Wissem (Verified Customer) (02-03-2026) | Antibody worked very well to immunoprecipitate RACGAP1( and KIF23) from cells arrested in G2/M, as well as to detect the protein in extracts
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