RACGAP1 Recombinant antibody

RACGAP1 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2D5

Cat no : 82788-1-RR

Synonyms

HsCYK 4, ID GAP, KIAA1478, Male germ cell RacGap, MgcRacGAP, RACGAP1

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Validation Data Gallery

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  WB analysis using 82788-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82788-1-RR (RACGAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 82788-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RACGAP1 antibody (82788-1-RR, Clone: 2D5 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, U2OS cells, K-562 cells, Jurkat cells
• Positive IF detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82788-1-RR targets RACGAP1 in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RACGAP1 fusion protein Ag4676
• Full Name: Rac GTPase activating protein 1
• Calculated Molecular Weight: 632 aa, 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC032754
• Gene Symbol: RACGAP1
• Gene ID (NCBI): 29127
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.