RAD1 Monoclonal antibody, PBS Only

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  WB analysis using 68637-1-Ig (same clone as 68637-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68637-1-Ig (RAD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68637-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 68637-1-Ig (same clone as 68637-1-PBS)

  IP result of anti-RAD1 (IP:68637-1-Ig, 4ug; Detection:68637-1-Ig 1:1000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 68637-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68637-1-Ig (same clone as 68637-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAD1 Monoclonal antibody (68637-1-Ig, Clone:1A11E2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68637-1-PBS in a different storage buffer formulation.

RAD1 Monoclonal Antibody for WB, FC (Intra), IP, Indirect ELISA

Cat No. 68637-1-PBS

Clone No.1A11E2

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, FC (Intra), IP, Indirect ELISA

Synonyms

REC1, Rad1-like DNA damage checkpoint protein, HRAD1, 1A11E2

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Product Information

68637-1-PBS targets RAD1 in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag33257

  Product name: Recombinant human RAD1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-282 aa of BC006837

  Sequence: MPLLTQQIQDEDDQYSLVASLDNVRNLSTILKAIHFREHATCFATKNGIKVTVENAKCVQANAFIQAGIFQEFKVQEESVTFRINLTVLLDCLSIFGSSPMPGTLTALRMCYQGYGYPLMLFLEEGGVVTVCKINTQEPEETLDFDFCSTNVINKIILQSEGLREAFSELDMTSEVLQITMSPDKPYFRLSTFGNAGSSHLDYPKDSDLMEAFHCNQTQVNRYKISLLKPSTKALVLSCKVSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEEVPESES

• Full Name: RAD1 homolog (S. pombe)
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC006837
• Gene Symbol: RAD1
• Gene ID (NCBI): 5810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60671
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RAD1 is also named as REC1,hRAD1. The human RAD1 and HUS1 proteins associate in a complex that interacts with a highly modified form of RAD9. They concluded that these 3 proteins are central components of a DNA damage-responsive protein complex in human cells.It has 3 isoforms produced by alternative splicing.