RAD23A Recombinant antibody

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  WB analysis of Jurkat using 86132-1-RR

  Various cell lysates were subjected to SDS PAGE followed by western blot with 86132-1-RR (RAD23A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

RAD23A Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86132-1-RR

Clone No.250716C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

HHR23A, HR23A

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Tested Applications

• Positive WB detected in: Jurkat cells, MCF-7 cells, SKOV-3 cells, OVCAR-3 cells, C6 cells, mouse testis tissue, rat testis cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86132-1-RR targets RAD23A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RAD23A fusion protein Ag0716
• Full Name: RAD23 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC014026
• Gene Symbol: RAD23A
• Gene ID (NCBI): 5886
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P54725
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAD23A, also named as HHR23A, belongs to the RAD23 family. It is a multiubiquitin chain receptor involved in modulation of proteasomal degradation. RAD23A binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. It is proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. It is involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC. It is also involved in vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages and is required for the association of HIV-1 vpr with the host proteasome.