Tested Applications
| Positive WB detected in | A431 cells, HEK-293 cells, HeLa cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12121-1-AP targets RAD23B in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2763 Product name: Recombinant human RAD23B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-409 aa of BC020973 Sequence: MQVTLKTLQQQTFKIDIDPEETVKALKEKIESEKGKDAFPVAGQKLIYAGKILNDDTALKEYKIDEKNFVVVMVTKPKAVSTPAPATTQQSAPASTTAVTSSTTTTVAQAPTPVPALAPTSTPASITPASATASSEPAPASAAKQEKPAEKPAETPVATSPTATDSTSGDSSRSNLFEDATSALVTGQSYENMVTEIMSMGYEREQVIAALRASFNNPDRAVEYLLMGIPGDRESQAVVDPPQAASTGVPQSSAVAAAAATTTATTTTTSSGGHPLEFLRNQPQFQQMRQIIQQNPSLLPALLQQIGRENPQLLQQISQHQEHFIQMLNEPVQEAGGQGGGGGGGSGGIAEAGSGHMNYIQVTPQEKEAIERLKALGFPEGLVIQAYFACEKNENLAANFLLQQNFDED Predict reactive species |
| Full Name | RAD23 homolog B (S. cerevisiae) |
| Calculated Molecular Weight | 409 aa, 43 kDa |
| Observed Molecular Weight | 58 kDa |
| GenBank Accession Number | BC020973 |
| Gene Symbol | RAD23B |
| Gene ID (NCBI) | 5887 |
| RRID | AB_10641048 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P54727 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAD23B, also named as p58, hHR23B and HR23B, belongs to the RAD23 family. It plays a central role both in proteosomal degradation of misfolded proteins and DNA repair. RAD23B is a central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in DNA excision repair by stabilizing XPC protein. It may play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes. RAD23B displays aberrant mobility in SDS-polyacrylamide gels, as it is typically detected as a 58- to 60-kDa protein despite a predicted size of 42 kDa (PMID: 8692695, 12052895).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RAD23B antibody 12121-1-AP | Download protocol |
| WB protocol for RAD23B antibody 12121-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
RAD23B antibody (Cat# 12121-1-AP) has 11 citations published in journals including Molecular Cell, International Journal of Biological Sciences, Neurotherapeutics, Frontiers in Immunology, Molecular & Cellular Proteomics, Clinical Proteomics, Frontiers in Oncology, Journal of Cancer, Neuroscience Research, PLoS ONE, and Journal of Biomedical Science. Associated research fields span cancer biology, neurodegeneration, proteasome-mediated protein degradation, ubiquitination, and DNA damage repair.
| Species | Application | Title |
|---|---|---|
Mol Cell RAD23B acquires a copper metalloadaptor function in amphibian-to-reptile evolution to increase metabolism and regulate genomic integrity | ||
Int J Biol Sci Deubiquitinase PSMD7 promotes the proliferation, invasion, and cisplatin resistance of gastric cancer cells by stabilizing RAD23B. | ||
Neurotherapeutics Rad23b exacerbates pathological aggregates through disrupting proteasome functions in Spinocerebellar ataxia type 3.
| ||
Front Immunol PDLIM7 Synergizes With PDLIM2 and p62/Sqstm1 to Inhibit Inflammatory Signaling by Promoting Degradation of the p65 Subunit of NF-κB. | ||
Mol Cell Proteomics The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators | ||
Clin Proteomics Investigation on the mechanisms of human sperm DNA damage based on the proteomics analysis by SWATH-MS |
