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RAD51AP1 Polyclonal antibody

RAD51AP1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 11255-1-AP

Synonyms

PIR51, RAD51 associated protein 1, RAD51 interacting protein, RAD51AP1



Tested Applications

Positive WB detected inHeLa cells, human brain tissue, mouse liver tissue, rat liver tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11255-1-AP targets RAD51AP1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAD51AP1 fusion protein Ag1781
Full Name RAD51 associated protein 1
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC016330
Gene symbol RAD51AP1
Gene ID (NCBI) 10635
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Homologous recombination (HR) is an important pathway for the elimination of DNA double-strand breaks (DSBs) and other deleterious lesions from chromosomes. The eukaryotic HR reaction is mediated by RAD51 [PMID:12456786]. RAD51AP1 (RAD51 associated protein 1), so named because it can interact with human RAD51, is a vertebrate-specific protein that enhances the ability of RAD51 to mediate the D-loop reaction. RAD51AP1 knockdown by siRNA leads to cellular sensitivity to DNA damaging agents and elevated DNA damage-induced chromatid breaks. Enhancement of the RAD51 recombinase activity by RAD51AP1 is contingent upon physical interaction of the two HR factors [PMID:17996710,17996711].

Protocols

Product Specific Protocols
WB protocol for RAD51AP1 antibody 11255-1-APDownload protocol
IF protocol for RAD51AP1 antibody 11255-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Biochem

The transcriptional regulator Zfat is essential for maintenance and differentiation of the adipocytes.

Authors - Shuhei Ishikura
humanWB

Oncogene

BRD4 inhibition sensitizes cervical cancer to radiotherapy by attenuating DNA repair.

Authors - Mengdong Ni
humanWB,IF

Nature

RNA transcripts stimulate homologous recombination by forming DR-loops.

Authors - Jian Ouyang
  • KD Validated