RAD9A Polyclonal antibody

• 

  WB analysis of HeLa using 13035-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13035-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13035-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 13035-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13035-1-AP (RAD9A antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13035-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13035-1-AP (RAD9A antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13035-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13035-1-AP (RAD9A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

RAD9A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13035-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RAD9, hRAD9, EC:3.1.11.2, Cell cycle checkpoint control protein RAD9A

---

Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, MCF-7 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

13035-1-AP targets RAD9A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3687

  Product name: Recombinant human RAD9A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 41-391 aa of BC014848

  Sequence: VNSSRSAYACFLFAPLFFQQYQAATPGQDLLRCKILMKSFLSVFRSLAMLEKTVEKCCISLNGRSSRLVVQLHCKFGVRKTHNLSFQDCASLQAVFDPASCPHMLRAPARVLGEAVLPFSPALAEVTLGIGRGRRVILRSYHEEEADSTAKAMVTEMCLGEEDFQQLQAQEGVAITFCLKEFRGLLSFAESANLNLSIHFDAPGRPAIFTIKDSLLDGHFVLATLSDTDSHSQDLGSPERHQPVPQLQAHSTPHPDDFANDDIDSYMIAMETTIGNEGSRVLPSISLSPGPQPPKSPGPHSEEEDEAEPSTVPGTPPPKKFRSLFFGSILAPVRSPQGPSPVLAEDSEGEG

• Full Name: RAD9 homolog A (S. pombe)
• Calculated Molecular Weight: 391 aa, 42 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC014848
• Gene Symbol: RAD9A
• Gene ID (NCBI): 5883
• RRID: AB_10598474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99638
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	EMBO J; YTHDC1 delays cellular senescence and pulmonary fibrosis by activating ATR in an m6A-independent manner; Authors - Canfeng Zhang
human	WB	Oncogene; DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.; Authors - Bin Chen
human	WB	J Mol Cell Biol; SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.; Authors - Xiaoli Xu
human	WB	DNA Cell Biol; AEG-1 Knockdown Sensitizes Glioma Cells to Radiation Through Impairing Homologous Recombination Via Targeting RFC5.; Authors - Xu Zhao
human	WB,IHC	Int Immunopharmacol; Unveiling an anoikis-related risk model and the role of RAD9A in colon cancer; Authors - Ting Yang ; KD Validated