RAD9A Polyclonal antibody

RAD9A Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 13035-1-AP

Synonyms

hRAD9, RAD9, RAD9 homolog A (S. pombe), RAD9A



Tested Applications

Positive WB detected inHeLa cells, A549 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

13035-1-AP targets RAD9A in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAD9A fusion protein Ag3687
Full Name RAD9 homolog A (S. pombe)
Calculated molecular weight 391 aa, 42 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC014848
Gene symbol RAD9A
Gene ID (NCBI) 5883
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAD9A antibody 13035-1-APDownload protocol
IHC protocol for RAD9A antibody 13035-1-APDownload protocol
IF protocol for RAD9A antibody 13035-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Mol Cell Biol

SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.

Authors - Xiaoli Xu