Tested Applications
| Positive WB detected in | BxPC-3 cells, HEK-293 cells, HepG2 cells, MCF-7 cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20491-1-AP targets RAE1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12102 Product name: Recombinant human RAE1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-368 aa of BC103754 Sequence: MSLFGTTSGFGTSGTSMFGSATTDNHNPMKDIEVTSSPDDSIGCLSFSPPTLPGNFLIAGSWANDVRCWEVQDSGQTIPKAQQMHTGPVLDVCWSDDGSKVFTASCDKTAKMWDLSSNQAIQIAQHDAPVKTIHWIKAPNYSCVMTGSWDKTLKFWDTRSSNPMMVLQLPERCYCADVIYPMAVVATAERGLIVYQLENQPSEFRRIESPLKHQHRCVAIFKDKQNKPTGFALGSIEGRVAIHYINPPNPAKDNFTFKCHRSNGTNTSAPQDIYAVNGIAFHPVHGTLATVGSDGRFSFWDKDARTKLKTSEQLDQPISACCFNHNGNIFAYASSYDWSKGHEFYNPQKKNYIFLRNAAEELKPRNKK Predict reactive species |
| Full Name | RAE1 RNA export 1 homolog (S. pombe) |
| Calculated Molecular Weight | 368 aa, 41 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | BC103754 |
| Gene Symbol | RAE1 |
| Gene ID (NCBI) | 8480 |
| RRID | AB_2878693 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78406 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAE1 acts as a mRNA export factor involved in nucleocytoplasmic transport, it plays a role in mitotic bipolar spindle formation. (PMID: 33849972, MID: 17172455)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RAE1 antibody 20491-1-AP | Download protocol |
| IHC protocol for RAE1 antibody 20491-1-AP | Download protocol |
| WB protocol for RAE1 antibody 20491-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Antibody 20491-1-AP (anti-RAE1) has 6 total citations published in Science, Cell Death & Disease, Developmental Cell, Cancer Science, Archives of Biochemistry and Biophysics, and iScience. Research fields span oocyte spindle assembly, lncRNA nuclear export, oligodendrocyte differentiation and CNS myelination, colorectal cancer, gastric carcinogenesis with EMT, and DNA repair linked to translation.
| Species | Application | Title |
|---|---|---|
Cell Death Discov Exon junction complex and nuclear pore complex components preferentially govern the nuclear export of spliced lncRNAs. | ||
Dev Cell Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination | ||
Arch Biochem Biophys RAE1 promotes gastric carcinogenesis and epithelial-mesenchymal transition | ||
iScience Integrative proteomics defines FANCA as a multifunctional hub linking DNA repair to translation. |
