RAE1 Polyclonal antibody

RAE1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 20491-1-AP

Synonyms

MIG14, Mnrp41, mRNA export factor, MRNP41, RAE1, Rae1 protein homolog



Tested Applications

Positive WB detected inBxPC-3 cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20491-1-AP is RAE1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAE1 fusion protein Ag12102
Full Name RAE1 RNA export 1 homolog (S. pombe)
Calculated molecular weight 368 aa, 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC103754
Gene symbol RAE1
Gene ID (NCBI) 8480
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAE1 antibody 20491-1-APDownload protocol
IHC protocol for RAE1 antibody 20491-1-APDownload protocol
IF protocol for RAE1 antibody 20491-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols