RAF1 Polyclonal antibody

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  IHC staining of human colon cancer using 12388-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12388-1-AP (RAF1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 12388-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12388-1-AP (RAF1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 12388-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12388-1-AP (RAF1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 12388-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12388-1-AP (RAF1 Antibody) at dilution of 1:50 (under 40x lens).

RAF1 Polyclonal Antibody for IHC, ELISA

Cat No. 12388-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Synonyms

c Raf, CRAF, NS5, Proto oncogene c RAF, RAF, Raf 1, RAF1

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Tested Applications

• Positive IHC detected in: human colon cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12388-1-AP targets RAF1 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3060

  Product name: Recombinant human RAF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 349-648 aa of BC018119

  Sequence: VMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF

• Full Name: v-raf-1 murine leukemia viral oncogene homolog 1
• Calculated Molecular Weight: 648 aa, 73 kDa
• GenBank Accession Number: BC018119
• Gene Symbol: RAF1
• Gene ID (NCBI): 5894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P04049
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.