Mouse RAGE Recombinant antibody, PBS Only

• 

  WB analysis using 83742-5-RR (same clone as 83742-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83742-5-RR (RAGE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83742-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 83742-5-RR (same clone as 83742-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83742-5-RR (Ager/Rage antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83742-5-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse lung using 83742-5-RR (same clone as 83742-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using Ager/Rage antibody (83742-5-RR, Clone: 240774F11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83742-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83742-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83608-4-RR against Human ATM were performed. The affinity constant is 2.58 nM. Biolayer interferometry (BLl) kinetic assays of 83608-4-RR against Mouse RAGE were performed. The affinity constant is 6.41 nM.

RAGE Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 83742-5-PBS

Clone No.240774F11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Ager, RAGE1, RAGE 1, MOK, 240774F11

---

Product Information

83742-5-PBS targets RAGE in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: advanced glycosylation end product-specific receptor
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 42-55 kDa
• GenBank Accession Number: NM_007425.3
• Gene Symbol: Ager
• Gene ID (NCBI): 11596
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q62151-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The receptor for advanced glycation end products (RAGE) is a cell surface transmembrane multiligand receptor, also known as Ager, is a multiligand member of the immunoglobulin superfamily. It has many isoforms and is predominantly expressed in the lung. AGER expression is upregulated in many diseases such as Alzheimer's disease, diabetes mellitus, atherosclerosis, and rheumatoid arthritis (PMID: 35099614).