Tested Applications
| Positive WB detected in | HeLa cells, human placenta tissue, mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17507-1-AP targets RAI14 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11708 Product name: Recombinant human RAI14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-347 aa of BC052988 Sequence: MKSLKAKFRKSDTNEWNKNDDRLLQAVENGDAEKVASLLGKKGASATKHDSEGKTAFHLAAAKGHVECLRVMITHGVDVTAQDTTGHSALHLAAKNSHHECIRKLLQSKCPAESVDSSGKTALHYAAAQGCLQAVQILCEHKSPINLKDLDGNIPLLLAVQNGHSEICHFLLDHGADVNSRNKSGRTALMLACEIGSSNAVEALIKKGADLNLVDSLGYNALHYSKLSENAGIQSLLLSKISQDADLKTPTKPKQHDQVSKISSERSGTPKKRKAPPPPISPTQLSDVSSPRSITSTPLSGKESVFFAEPPFKAEISSIRENKDRLSDSTTGADSLLDISSEADQQD Predict reactive species |
| Full Name | retinoic acid induced 14 |
| Calculated Molecular Weight | 980 aa, 110 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC052988 |
| Gene Symbol | RAI14 |
| Gene ID (NCBI) | 26064 |
| RRID | AB_2175992 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P0K7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RAI14 antibody 17507-1-AP | Download protocol |
| IHC protocol for RAI14 antibody 17507-1-AP | Download protocol |
| IP protocol for RAI14 antibody 17507-1-AP | Download protocol |
| WB protocol for RAI14 antibody 17507-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RAI14 Polyclonal antibody (Cat# 17507-1-AP) has 13 citations across journals including Genes Dev, EMBO Rep, Mol Cell Proteomics, Cells, Cell Signal, Int J Mol Sci, Front Pharmacol, Cell Transplant, PeerJ, PLoS One, Onco Targets Ther, Elife, and bioRxiv. Research fields span oncology (colon, melanoma, breast, gastric cancer), renal fibrosis, atherogenesis, neuroscience (dendritic spines, depression), reproductive biology (spermatogenesis), and mechanotransduction/Hippo signaling.
| Species | Application | Title |
|---|---|---|
Genes Dev Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein | ||
Mol Cell Proteomics Phenotypic heterogeneity analysis of APC-mutant colon cancer by proteomics and phosphoproteomics identifies RAI14 as a key prognostic determinant in East Asians and Westerners
| ||
Cells Cysteine-Rich LIM-Only Protein 4 (CRP4) Promotes Atherogenesis in the ApoE-/- Mouse Model. | ||
Int J Mol Sci RAI14 Promotes Melanoma Progression by Regulating the FBXO32/c-MYC Pathway
| ||
Front Pharmacol High Expression of RAI14 in Triple-Negative Breast Cancer Participates in Immune Recruitment and Implies Poor Prognosis Through Bioinformatics Analyses. |
