RAI14 Recombinant monoclonal antibody

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  WB analysis using 86581-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86581-1-RR (RAI14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 86581-1-RR

  rat testis tissue were subjected to SDS PAGE followed by western blot with 86581-1-RR (RAI14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86581-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAI14 antibody (86581-1-RR, Clone: 251495G12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86581-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86581-1-RR against Human RAI14 were performed. The affinity constant is 7.73 nM.

RAI14 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86581-1-RR

Clone No.251495G12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Ankycorbin, Ankyrin repeat and coiled-coil structure-containing protein, KIAA1334, NORPEG, RAI13

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Tested Applications

• Positive WB detected in: HeLa cells, rat testis tissue, HEK-293T cells, NIH/3T3 cells, mouse testis tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86581-1-RR targets RAI14 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag11708

  Product name: Recombinant human RAI14 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-347 aa of BC052988

  Sequence: MKSLKAKFRKSDTNEWNKNDDRLLQAVENGDAEKVASLLGKKGASATKHDSEGKTAFHLAAAKGHVECLRVMITHGVDVTAQDTTGHSALHLAAKNSHHECIRKLLQSKCPAESVDSSGKTALHYAAAQGCLQAVQILCEHKSPINLKDLDGNIPLLLAVQNGHSEICHFLLDHGADVNSRNKSGRTALMLACEIGSSNAVEALIKKGADLNLVDSLGYNALHYSKLSENAGIQSLLLSKISQDADLKTPTKPKQHDQVSKISSERSGTPKKRKAPPPPISPTQLSDVSSPRSITSTPLSGKESVFFAEPPFKAEISSIRENKDRLSDSTTGADSLLDISSEADQQD

• Full Name: retinoic acid induced 14
• Calculated Molecular Weight: 980 aa, 110 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC052988
• Gene Symbol: RAI14
• Gene ID (NCBI): 26064
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9P0K7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAI14 (retinoic acid-induced protein 14, also called ankycorbin or NORPEG) was originally identified in human retinal pigment epithelial cells as a retinoic-acid-responsive gene. Through direct interaction with actin-binding partners such as palladin and TARA, RAI14 orchestrates focal adhesion dynamics, cell polarity and mechano-sensing; in epithelia it stabilizes tight-junction complexes, whereas in neurons it localizes to the dendritic-spine neck where it promotes spine maintenance by antagonizing F-actin depolymerization. Consistent with its cytoskeletal role, RAI14 has emerged as a context-dependent modulator of several signalling cascades.