RALGAPB Polyclonal antibody

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  WB analysis using 28330-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28330-1-AP (RALGAPB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 28330-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28330-1-AP (RALGAPB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 28330-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28330-1-AP (RALGAPB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28330-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28330-1-AP (RALGAPB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 28330-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28330-1-AP (RALGAPB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RALGAPB Polyclonal Antibody for WB, IHC, ELISA

Cat No. 28330-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

WB, IHC, ELISA

Synonyms

KIAA1219, p170, RALGAPB

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, rat brain
• Positive IHC detected in: human liver cancer tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

28330-1-AP targets RALGAPB in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28259

  Product name: Recombinant human KIAA1219 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1222-1494 aa of BC047482

  Sequence: NCCDDGEGSQQEVISSEDIGASIFNGQKKVLYYADALTEIAFVVPSPVESLTDSLESNISDQDSDSNMDLMPGILKQPSLTLELFPNHTDNLNSSQRLSPSSRMRKLPQGRPVPPLGPETRVSVVWVERYDDIENFPLSELMTEISTGVETTANSSTSLRSTTLEKEVPVIFIHPLNTGLFRIKIQGATGKFNMVIPLVDGMIVSRRALGFLVRQTVINICRRKRLESDSYSPPHVRRKQKITDIVNKYRNKQLEPEFYTSLFQEVGLKNCSS

• Full Name: KIAA1219
• Observed Molecular Weight: 160-170 kDa
• GenBank Accession Number: BC047482
• Gene Symbol: RALGAPB
• Gene ID (NCBI): 57148
• RRID: AB_2918155
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86X10
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Nat Commun; Structure and mechanism of the RalGAP tumor suppressor complex; Authors - René Rasche
mouse	WB	Life Sci Alliance; RalGAP complexes control secretion and primary cilia in pancreatic disease; Authors - Lisa H Apken