RALY Polyclonal antibody

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RALY Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 17896-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, RIP, ELISA

Synonyms

Autoantigen p542, Heterogeneous nuclear ribonucleoprotein C-like 2, hnRNP associated with lethal yellow protein homolog, hnRNP core protein C-like 2, HNRPCL2

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  WB analysis using 17896-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17896-1-AP (RALY antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HEK-293 using 17896-1-AP

  WB result of RALY antibody (17896-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RALY transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IP experiment of HEK-293T using 17896-1-AP

  IP result of anti-RALY (IP:17896-1-AP, 4ug; Detection:17896-1-AP 1:8000) with HEK-293T cells lysate 2200 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of mouse lung using 17896-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 17896-1-AP (RALY antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human pancreas cancer using 17896-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17896-1-AP (RALY antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human placenta using 17896-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17896-1-AP (RALY antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of mouse pancreas using 17896-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17896-1-AP (RALY antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of mouse small intestine using 17896-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 17896-1-AP (RALY antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat pancreas using 17896-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 17896-1-AP (RALY antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat small intestine using 17896-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 17896-1-AP (RALY antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells
• Positive IP detected in: HEK-293T cells
• Positive IHC detected in: human pancreas cancer tissue, mouse lung tissue, rat pancreas tissue, mouse pancreas tissue, mouse small intestine tissue, human placenta tissue, rat small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17896-1-AP targets RALY in WB, IHC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12250

  Product name: Recombinant human RALY protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-307 aa of BC103753

  Sequence: MSLKLQASNVTNKNDPKSINSRVFIGNLNTALVKKSDVETIFSKYGRVAGCSVHKGYAFVQYSNERHARAAVLGENGRVLAGQTLDINMAGEPKPDRPKGLKRAASAIYSGYIFDYDYYRDDFYDRLFDYRGRLSPVPVPRAVPVKRPRVTVPLVRRVKTNVPVKLFARSTAVTTSSAKIKLKSSELQAIKTELTQIKSNIDALLSRLEQIAAEQKANPDGKKKGDGGGASGGGGGGGGSGGGGSGGGGGGGSSRPPAPQENTTSEAGLPQGEARTRDDGDEEGLLTHSEEELEHSQDTDADDGALQ

• Full Name: RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse))
• Calculated Molecular Weight: 307 aa, 33 kDa
• Observed Molecular Weight: 34-36 kDa
• GenBank Accession Number: BC103753
• Gene Symbol: RALY
• Gene ID (NCBI): 22913
• RRID: AB_3085544
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UKM9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RALY Polyclonal antibody (Cat# 17896-1-AP) has 1 total citation. It appears in Cell Death Discovery (IF 10.4), published in 2026. The cited study investigates how tRF-3005a regulates exon skipping of SPAG4 by interacting with RALY to drive gastric cancer progression, using WB and RIP applications in human samples. The research field is gastric cancer biology and RNA-mediated gene regulation.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,RIP	Cell Death Discov; tRF-3005a regulates exon skipping of SPAG4 by interacting with RALY to drive gastric cancer progression.; Authors - Huaiping Cui