Featured Product
KD/KO Validated

RAN Recombinant monoclonal antibody

RAN Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 82978-1-RR

Clone No. 230317F3

Publications (1)

Review Product

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

ARA24, 230317F3, Androgen receptor-associated protein 24, EC:3.6.5.-, GTP binding nuclear protein Ran

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIHCIF/ICCFC (Intra)Affinity

WB analysis using 82978-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 82978-1-RR (RAN antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 82978-1-RR
WB result of RAN antibody (82978-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAN transfected HEK-293 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat kidney using 82978-1-RR
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 82978-1-RR (RAN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 82978-1-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82978-1-RR (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 82978-1-RR
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82978-1-RR (RAN antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 82978-1-RR
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82978-1-RR (RAN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 82978-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAN antibody (82978-1-RR, Clone: 230317F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of A431 using 82978-1-RR
1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human RAN (82978-1-RR, Clone:230317F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of HeLa using 82978-1-RR
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human RAN (82978-1-RR, Clone:230317F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 82978-1-RR
Biolayer interferometry (BLl) kinetic assays of 82978-1-RR against Human RAN were performed. The affinity constant is 3.08 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for RAN in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, A375 cells, Jurkat cells, NIH/3T3 cells, C6 cells
Positive IHC detected in	human colon tissue, mouse kidney tissue, rat kidney tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells, A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82978-1-RR targets RAN in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag0748 Product name: Recombinant human RAN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC004272 Sequence: MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL Predict reactive species
Full Name	RAN, member RAS oncogene family
Calculated Molecular Weight	25 kDa
Observed Molecular Weight	24 kDa
GenBank Accession Number	BC004272
Gene Symbol	RAN
Gene ID (NCBI)	5901
RRID	AB_3670726
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P62826
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAN is also named as ARA24, Ras-like protein TC4 and GTPase Ran, and belongs to the small GTPase superfamily family and the Ran family. GTPase Ran switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PMID:7819259, PMID:32528950). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PMID:10408446, PMID:29040603, PMID:32528950).

Protocols

Product Specific Protocols
IF protocol for RAN antibody 82978-1-RR	Download protocol
IHC protocol for RAN antibody 82978-1-RR	Download protocol
WB protocol for RAN antibody 82978-1-RR	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The RAN Recombinant monoclonal antibody (230317F3), catalog number 82978-1-RR, has 1 total citation. The cited publication appeared in Advanced Biology (Adv Biol, Weinh) and investigated RAN's role in promoting autophagy and malignant progression of lung adenocarcinoma through ATG101, placing the research in the fields of cancer biology and autophagy. The antibody was applied in IHC on human samples.