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RANBP10 Polyclonal antibody

RANBP10 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21107-1-AP

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

KIAA1464, RAN binding protein 10, RANBP10

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of MCF-7 using 21107-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 21107-1-AP (RANBP10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse brain using 21107-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21107-1-AP (RANBP10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse heart using 21107-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 21107-1-AP (RANBP10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of LNCaP using 21107-1-AP
LNCaP cells were subjected to SDS PAGE followed by western blot with 21107-1-AP (RANBP10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse skeletal muscle using 21107-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21107-1-AP (RANBP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human hepatocirrhosis using 21107-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21107-1-AP (RANBP10 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human hepatocirrhosis using 21107-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21107-1-AP (RANBP10 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 21107-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 21107-1-AP (RANBP10 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 21107-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 21107-1-AP (RANBP10 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	MCF-7 cells, mouse skeletal muscle tissue, mouse heart tissue, LNCaP cells, mouse brain tissue
Positive IHC detected in	human hepatocirrhosis tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21107-1-AP targets RANBP10 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15387 Product name: Recombinant human RANBP10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 362-497 aa of BC099917 Sequence: YPGSPSLSPRHGPSSSHMHNTGADSPSCSNGVASTKSKQNHSKYPAPSSSSSSSSSSSSSSPSSVNYSESNSTDSTKSQHHSSTSNQETSDSEVEMEAEHYPNGVLGSMSTRIVNGAYKHEDLQTDESSMDDRHPR Predict reactive species
Full Name	RAN binding protein 10
Calculated Molecular Weight	620 aa, 67 kDa
Observed Molecular Weight	67-70 kDa
GenBank Accession Number	BC099917
Gene Symbol	RANBP10
Gene ID (NCBI)	57610
RRID	AB_10732603
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6VN20
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for RANBP10 antibody 21107-1-AP	Download protocol
WB protocol for RANBP10 antibody 21107-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The RANBP10 Polyclonal antibody (Cat# 21107-1-AP) has 3 citations published in Theranostics, PLoS Pathogens, and bioRxiv. The research fields span neuroprotection in Huntington's disease (miR-196a/RANBP10 axis), human papillomavirus type 16 nuclear import mechanisms, and ribosome biogenesis linked to CTLH E3 ligase complex regulation. Applications cited include WB, IF, and IP across human and mouse species.

Species	Application	Title
mouse	WB,IF	Theranostics miR-196a Enhances Neuronal Morphology through Suppressing RANBP10 to Provide Neuroprotection in Huntington's Disease. Authors - Lu-Shiun Her View Article
human	WB	PLoS Pathog A Ran-binding protein facilitates nuclear import of human papillomavirus type 16. Authors - Kun-Yi Lai KD Validated View Article
human	WB,IP	bioRxiv Proteome-wide C-degron activity profiling connects conditional regulation of the CTLH E3 ligase complex to ribosome biogenesis. Authors - Drew W. Grant View Article