RANBP3L Polyclonal antibody

RANBP3L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17875-1-AP

Synonyms

RAN binding protein 3 like, RANBP3L

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Validation Data Gallery

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  WB analysis using 17875-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17875-1-AP (RANBP3L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17875-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17875-1-AP (RANBP3L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17875-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17875-1-AP (RANBP3L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 17875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17875-1-AP (RANBP3L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 17875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17875-1-AP (RANBP3L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17875-1-AP (RANBP3L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17875-1-AP (RANBP3L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: mouse kidney tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17875-1-AP targets RANBP3L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RANBP3L fusion protein Ag11642
• Full Name: RAN binding protein 3-like
• Calculated Molecular Weight: 465 aa, 52 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC047660
• Gene Symbol: RANBP3L
• Gene ID (NCBI): 202151
• RRID: AB_2284894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.