RANBP9 Polyclonal antibody

RANBP9 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17755-1-AP

Synonyms

BPM L, BPM90, RAN binding protein 9, Ran binding protein M, RanBP7, RANBP9, RANBPM



Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, human testis tissue, mouse testis tissue, rat testis tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, mouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17755-1-AP targets RANBP9 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Reactivity human, mouse, rat, Canine
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RANBP9 fusion protein Ag12112
Full Name RAN binding protein 9
Calculated molecular weight388aa,43 kDa; 729aa,78 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC063849
Gene symbol RANBP9
Gene ID (NCBI) 10048
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RANBP9 antibody 17755-1-APDownload protocol
IHC protocol for RANBP9 antibody 17755-1-APDownload protocol
IF protocol for RANBP9 antibody 17755-1-APDownload protocol
IP protocol for RANBP9 antibody 17755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Acta Histochem

MicroRNA-26a-5p alleviates neuronal apoptosis and brain injury in intracerebral hemorrhage by targeting RAN binding protein 9.

Authors - Huiyuan Zhang
humanWB

Oncol Lett

RANBP9 suppresses tumor proliferation in colorectal cancer.

Authors - Chunzhi Qin