RanGAP1 Polyclonal antibody

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  WB analysis using 11102-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11102-1-AP (RANGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11102-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11102-1-AP (RANGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11102-1-AP

  IP result of anti-RANGAP1 (IP:11102-1-AP, 4ug; Detection:11102-1-AP 1:300) with HEK-293 cells lysate 3600ug.

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  IHC staining of human gliomas using 11102-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11102-1-AP (RANGAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11102-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11102-1-AP (RANGAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11102-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11102-1-AP (RANGAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 11102-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RANGAP1 antibody (11102-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

• Featured Product
• KD/KO Validated

RanGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11102-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

KIAA1835, Ran GTPase-activating protein 1, SD

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

11102-1-AP targets RanGAP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RanGAP1 fusion protein Ag1563
• Full Name: Ran GTPase activating protein 1
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC014044
• Gene Symbol: RANGAP1
• Gene ID (NCBI): 5905
• RRID: AB_2176968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P46060
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF,CoIP	Cell Oncol (Dordr); Hepatitis B virus core protein stabilizes RANGAP1 to upregulate KDM2A and facilitate hepatocarcinogenesis; Authors - Hong-Juan You
human	WB	J Proteomics; mTORC1 alters the expression of glycolytic genes by regulating KPNA2 abundances.; Authors - Xianwei Chen
human,mouse	WB,IHC,IP	Dev Cell; Loss of RanGAP1 drives chromosome instability and rapid tumorigenesis of osteosarcoma; Authors - Yan Gong
human	WB,IHC,IP	Cancer Gene Ther; METTL3-mediated RanGAP1 promotes colorectal cancer progression through the MAPK pathway by recruiting YTHDF1; Authors - Rui Yang ; KD Validated