RANGRF Polyclonal antibody

RANGRF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10552-1-AP

Synonyms

RanGNRF, Ran-binding protein MOG1

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Validation Data Gallery

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  WB analysis of SGC-7901 using 10552-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10552-1-AP (RANGRF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10552-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10552-1-AP (RANGRF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10552-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10552-1-AP (RANGRF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 10552-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10552-1-AP (RANGRF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 10552-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10552-1-AP (RANGRF antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10552-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10552-1-AP (RANGRF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10552-1-AP

  Immunofluorescent analysis of HepG2 cells using 10552-1-AP (RANGRF antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SGC-7901 cells, HepG2 cells, mouse lung tissue, mouse testis tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

10552-1-AP targets RANGRF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RANGRF fusion protein Ag0824
• Full Name: RAN guanine nucleotide release factor
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18-22 kDa
• GenBank Accession Number: BC006486
• Gene Symbol: RANGRF
• Gene ID (NCBI): 29098
• RRID: AB_2238173
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RANGRF, also named as MOG1, RANGNRF, HSPC165, HSPC236 and MDS5, regulates the intracellular trafficking of RAN. In cardiac cells it regulates the cell surface localization of SCN5A.

Publications

Species	Application	Title
mouse	IHC	Sci Transl Med; Gene therapy targeting protein trafficking regulator MOG1 in mouse models of Brugada syndrome, arrhythmias, and mild cardiomyopathy.; Authors - Gang Yu
		Stem Cell Res; Generation of a homozygous RANGRF knockout hiPSC line by CRISPR/Cas9 system; Authors - Meiling Jiang