RAP1A Recombinant monoclonal antibody, PBS Only

Name: RAP1A Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 83708-1-PBS-100UG

RAP1A Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 83708-1-PBS

Clone No.240438G5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

240438G5, EC:3.6.5.2, G-22K, GTP-binding protein smg p21A, KREV1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

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Filter: AllWBIF/ICCFC (Intra)Affinity

WB analysis using 83708-1-RR (same clone as 83708-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83708-1-RR (RAP1A antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of A431 using 83708-1-RR (same clone as 83708-1-PBS)
WB result of RAP1A antibody (83708-1-RR; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAP1A transfected A431 cells. This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of NIH/3T3 using 83708-1-RR (same clone as 83708-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RAP1A antibody (83708-1-RR, Clone: 240438G5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of HeLa using 83708-1-RR (same clone as 83708-1-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAP1A Recombinant antibody (83708-1-RR, Clone:240438G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAP1A Recombinant antibody (83708-1-RR, Clone:240438G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 83708-1-RR
Biolayer interferometry (BLl) kinetic assays of 83708-1-RR against Human RAP1A were performed. The affinity constant is below 1 pM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

83708-1-PBS targets RAP1A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag9488 Product name: Recombinant human RAP1A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-184 aa of BC014086 Sequence: MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKKPKKKSCLLL Predict reactive species
Full Name	RAP1A, member of RAS oncogene family
Calculated Molecular Weight	184 aa, 21 kDa
GenBank Accession Number	BC014086
Gene Symbol	RAP1A
Gene ID (NCBI)	5906
RRID	AB_3671310
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	P62834
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

RAP1A(Ras-related protein Rap-1A), also known as KREV1. Induces morphological reversion of a cell line transformed by a Ras oncogene. The biological roles of RAP1A include inducing neurite outgrowth and embryonic angiogenesis(PMID: 17916086). As one member of RAS oncogene family, it is a widely expressed receptor,with high mRNA expression in many tissues, particularly abundant in the colon. In cells, RAP1A was localized in cytoplasm and cell membrane(PMID: 11359771, PMID: 11359771).