RAP1A Recombinant antibody, PBS Only

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  WB analysis using 83708-1-RR (same clone as 83708-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83708-1-RR (RAP1A antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 83708-1-RR (same clone as 83708-1-PBS)

  WB result of RAP1A antibody (83708-1-RR; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAP1A transfected A431 cells. This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 83708-1-RR (same clone as 83708-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RAP1A antibody (83708-1-RR, Clone: 240438G5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83708-1-RR (same clone as 83708-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAP1A Recombinant antibody (83708-1-RR, Clone:240438G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83708-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83708-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83708-1-RR against Human RAP1A were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

RAP1A Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 83708-1-PBS

Clone No.240438G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

240438G5, EC:3.6.5.2, G-22K, GTP-binding protein smg p21A, KREV1

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Product Information

83708-1-PBS targets RAP1A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RAP1A fusion protein Ag9488
• Full Name: RAP1A, member of RAS oncogene family
• Calculated Molecular Weight: 184 aa, 21 kDa
• GenBank Accession Number: BC014086
• Gene Symbol: RAP1A
• Gene ID (NCBI): 5906
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P62834
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RAP1A(Ras-related protein Rap-1A), also known as KREV1. Induces morphological reversion of a cell line transformed by a Ras oncogene. The biological roles of RAP1A include inducing neurite outgrowth and embryonic angiogenesis(PMID: 17916086). As one member of RAS oncogene family, it is a widely expressed receptor,with high mRNA expression in many tissues, particularly abundant in the colon. In cells, RAP1A was localized in cytoplasm and cell membrane(PMID: 11359771, PMID: 11359771).