RAP1GDS1 Polyclonal antibody

RAP1GDS1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 10377-1-AP

Synonyms

Exchange factor smgGDS, GDS1, RAP1GDS1, SMG GDS protein, SmgGDS

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Validation Data Gallery

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  WB analysis of mouse brain using 10377-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10377-1-AP (RAP1GDS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10377-1-AP

  IP result of anti-RAP1GDS1 (IP:10377-1-AP, 3ug; Detection:10377-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 10377-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10377-1-AP (RAP1GDS1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 10377-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10377-1-AP (RAP1GDS1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

10377-1-AP targets RAP1GDS1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAP1GDS1 fusion protein Ag0230
• Full Name: RAP1, GTP-GDP dissociation stimulator 1
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC001851
• Gene Symbol: RAP1GDS1
• Gene ID (NCBI): 5910
• RRID: AB_2284927
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GDS stimulates the GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins), including at least c-Ki-ras p21, smg p21A, smg p21B, rhoA p21 and rhoB p21, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein. The small GTPase Rap1, which is activated by a large variety of stimuli, functions in the control of integrin-mediated cell adhesion. RAP1GDS1 (synonym: GDS1)is one of RAP1 GTP-GDP dissociation stimulators.

Publications

Species	Application	Title
	IP	Nat Genet; Mutations in RABL3 alter KRAS prenylation and are associated with hereditary pancreatic cancer.; Authors - Sahar Nissim
chicken	WB,IF	Microbiol Spectr; Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.; Authors - Beibei Zhang
human	WB	Oncol Lett; SUMO4 small interfering RNA attenuates invasion and migration via the JAK2/STAT3 pathway in non-small cell lung cancer cells.; Authors - Jin-Xiao Liang
human,mouse	WB	Commun Biol; Vimar/RAP1GDS1 promotes acceleration of brain aging after flies and mice reach middle age; Authors - Ying Xiong