RAP1GDS1 Monoclonal antibody

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• RAP1GDS1 Monoclonal antibody (60940-1-Ig)

• 

  WB analysis using 60940-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60940-1-Ig (RAP1GDS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

RAP1GDS1 Monoclonal Antibody for WB, ELISA

Cat No. 60940-1-Ig

Clone No.1E4H12

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

Exchange factor smgGDS, Rap1 GTPase-GDP dissociation stimulator 1, SMG GDS protein, SmgGDS

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Tested Applications

• Positive WB detected in: LNCaP cells, Jurkat cells, K-562 cells, MOLT-4 cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60940-1-Ig targets RAP1GDS1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag32728

  Product name: Recombinant human RAP1GDS1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 176-406 aa of BC001851

  Sequence: QLINMGVIPTLVKLLGIHCQNAALTEMCLVAFGNLAELESSKEQFASTNIAEELVKLFKKQIEHDKREMIFEVLAPLAENDAIKLQLVEAGLVECLLEIVQQKVDSDKEDDITELKTGSDLMVLLLLGDESMQKLFEGGKGSVFQRVLSWIPSNNHQLQLAGALAIANFARNDANCIHMVDNGIVEKLMDLLDRHVEDGNVTVQHAALSALRNLAIPVINKAKMLSAGVTE

• Full Name: RAP1, GTP-GDP dissociation stimulator 1
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 55-66 kDa
• GenBank Accession Number: BC001851
• Gene Symbol: RAP1GDS1
• Gene ID (NCBI): 5910
• RRID: AB_3743250
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P52306
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GDS stimulates the GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins), including at least c-Ki-ras p21, smg p21A, smg p21B, rhoA p21 and rhoB p21, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein. The small GTPase Rap1, which is activated by a large variety of stimuli, functions in the control of integrin-mediated cell adhesion. RAP1GDS1 (synonym: GDS1)is one of RAP1 GTP-GDP dissociation stimulators.