Tested Applications
| Positive WB detected in | A431 cells, mouse lung tissue, K-562 cells, mouse brain tissue, PC-3 cells, HeLa cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13789-1-AP targets RAP2A in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4808 Product name: Recombinant human RAP2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-183 aa of BC041333 Sequence: MREYKVVVLGSGGVGKSALTVQFVTGTFIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIIRVKRYEKVPVILVGNKVDLESEREVSSSEGRALAEEWGCPFMETSAKSKTMVDELFAEIVRQMNYAAQPDKDDPCCSACNIQ Predict reactive species |
| Full Name | RAP2A, member of RAS oncogene family |
| Calculated Molecular Weight | 183 aa, 21 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC041333 |
| Gene Symbol | RAP2A |
| Gene ID (NCBI) | 5911 |
| RRID | AB_2177303 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10114 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RAP2A antibody 13789-1-AP | Download protocol |
| WB protocol for RAP2A antibody 13789-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RAP2A antibody (Cat# 13789-1-AP) has 3 total citations across three journals: Advanced Science, Scientific Reports, and Clinical and Experimental Medicine. Research fields span pulmonary fibrosis and immune microenvironment regulation (MAP4K4-VCAM1 signaling), neuronal dendritic branching via the AKT/GSK3β pathway, and hepatocellular carcinoma apoptosis resistance through the mTOR pathway. Applications include CoIP, WB, and IHC in human samples.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Inactivation Rap2a in Endothelial Cell Prevents Pulmonary Fibrosis by Regulating Immune Microenvironment Through MAP4K4-VCAM1 Signaling. | ||
Sci Rep miR-9 and miR-124 synergistically affect regulation of dendritic branching via the AKT/GSK3β pathway by targeting Rap2a. | ||
Clin Exp Med RAP2A promotes apoptosis resistance of hepatocellular carcinoma cells via the mTOR pathway. |
Reviews
What customers say
The single review for this product was a 1-star rating from a Western Blot application on MDCK GII cells at a 1:1,000 dilution. The reviewer reported that RAP2A should appear at approximately 21 kD, but no prominent bands were observed at that expected molecular weight, indicating the antibody did not perform as anticipated in their experiment.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Joleen (Verified Customer) (05-29-2019) | RAP2A should be 21kD, no prominent bands appeared at that molecular weight.
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