EPAC2 Polyclonal antibody

EPAC2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 19103-1-AP

Synonyms

CAMP GEFII, CGEF2, EPAC 2, EPAC2, Nbla00496, RAPGEF4

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Validation Data Gallery

• 

  WB analysis of mouse brain using 19103-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19103-1-AP (EPAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 19103-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 19103-1-AP (EPAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 19103-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19103-1-AP (EPAC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 19103-1-AP

  IP result of anti-EPAC2 (IP:19103-1-AP, 4ug; Detection:19103-1-AP 1:600) with mouse brain tissue lysate 2560ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue, HepG2 cells
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

19103-1-AP targets EPAC2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EPAC2 fusion protein Ag5186
• Full Name: Rap guanine nucleotide exchange factor (GEF) 4
• Calculated Molecular Weight: 116 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC024004
• Gene Symbol: EPAC2/RAPGEF4
• Gene ID (NCBI): 11069
• RRID: AB_10597393
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	J Cell Mol Med; Differential changes in cyclic adenosine 3'-5' monophosphate (cAMP) effectors and major Ca2+ handling proteins during diabetic cardiomyopathy; Authors - Victoria Chaoul
rat	WB,IF	J Diabetes Investig; Epac2 activation mediates glucagon-induced glucogenesis in primary rat hepatocytes; Authors - Yusuke Shiozaki-Takagi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This EPAC2 antibody successfully detected a strong EPAC2 band in mouse heart lysate, which is about 120 kDa.

• Applications: Western Blot
• Cell Tissue Type: Heart