Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue, HepG2 cells |
| Positive IP detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19103-1-AP targets EPAC2 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5186 Product name: Recombinant human RAPGEF4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 712-1011 aa of BC024004 Sequence: VKMSSGGEKVVLKPNDVSVFMTLTINGRLFACPREQFDSLTPLPEQEGPTVGTVGTFELMSSKDLAYQMTIYDWELFNCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYRLTVAKLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTARTVRYYRSQPFNPDAAQANKNHQDVRSYVRQLNVIDNQRTLSQMSHRLEPRRP Predict reactive species |
| Full Name | Rap guanine nucleotide exchange factor (GEF) 4 |
| Calculated Molecular Weight | 116 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC024004 |
| Gene Symbol | EPAC2/RAPGEF4 |
| Gene ID (NCBI) | 11069 |
| RRID | AB_10597393 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WZA2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for EPAC2 antibody 19103-1-AP | Download protocol |
| WB protocol for EPAC2 antibody 19103-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-EPAC2 antibody (Cat# 19103-1-AP) has 5 citations spanning Communications Biology, Journal of Cellular and Molecular Medicine, Viruses, Journal of Diabetes Investigation, and bioRxiv. Research fields include LDL cholesterol clearance, diabetic cardiomyopathy, respiratory syncytial virus infection, glucagon-induced glucogenesis in hepatocytes, and retinal neurogenesis in ocular albinism. Applications used were primarily Western blot, with one IP and one IF. Species covered include mouse, rat, and human.
| Species | Application | Title |
|---|---|---|
Commun Biol ABCC4 impairs the clearance of plasma LDL cholesterol through suppressing LDLR expression in the liver.
| ||
J Cell Mol Med Differential changes in cyclic adenosine 3'-5' monophosphate (cAMP) effectors and major Ca2+ handling proteins during diabetic cardiomyopathy | ||
Viruses The Impact of EPAC2-Associated Junction Plakoglobin on Respiratory Syncytial Virus Infection | ||
J Diabetes Investig Epac2 activation mediates glucagon-induced glucogenesis in primary rat hepatocytes | ||
bioRxiv Decreased CREB phosphorylation impairs embryonic retinal neurogenesis in the Oa1-/- mouse model of Ocular albinism. |
Reviews
What customers say
Both reviewers gave the antibody a perfect 5-star rating. One user highlighted that it successfully detected a strong EPAC2 band at approximately 120 kDa in mouse heart lysate by Western Blot. The other praised Proteintech for consistently delivering high-quality antibodies at reasonable prices, calling it their lab's first-choice supplier. Overall, customers report reliable performance and good value for this product.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Xuejun (Verified Customer) (08-03-2026) | Proteintech consistently provides high-quality antibodies at reasonable prices, making it our lab’s first-choice supplier.
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Tongbin (Verified Customer) (08-25-2020) | This EPAC2 antibody successfully detected a strong EPAC2 band in mouse heart lysate, which is about 120 kDa.
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