RARG Polyclonal antibody

• Featured Product
• KD/KO Validated

RARG Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ChIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11424-1-AP

Synonyms

NR1B3, RAR gamma, RARC, RARG, Retinoic acid receptor gamma, retinoic acid receptor, gamma

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Validation Data Gallery

• 

  WB analysis of HeLa using 11424-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11424-1-AP (RARG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11424-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11424-1-AP (RARG Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 11424-1-AP

  IP result of anti-RARG (IP:11424-1-AP, 4ug; Detection:11424-1-AP 1:300) with MCF-7 cells lysate 1120ug.

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 2 publications below

Product Information

11424-1-AP targets RARG in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RARG fusion protein Ag1991
• Full Name: retinoic acid receptor, gamma
• Calculated Molecular Weight: 454 aa, 50 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC019098
• Gene Symbol: RARG
• Gene ID (NCBI): 5916
• RRID: AB_2175394
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Retinoic acid receptors (RARs) are nuclear hormone receptors that act as ligand-dependent transcriptional regulators. In their liganded state, RARs activate transcription, whereas in their nonliganded form, they repress transcription of their target genes. RARs have numerous target genes, which have retinoic response elements in their promoter regions [PMID:17574023]. RARG is a member of the retinoid acid receptor (RAR) family, along with RARA, which is known to be involved PML/RARA fusion of acute promyelocytic leukemia, and RARB. and rearrangement that conferred to leukemic cells acute promyelocytic leukemia-like morphologic and immunophenotypic features [PMID:20935257].

Publications

Species	Application	Title
mouse	ChIP	J Clin Invest; The orphan nuclear receptor small heterodimer partner mediates male infertility induced by diethylstilbestrol in mice.; Authors - Volle David H DH
human	WB,IHC	Front Oncol; High expression of RARG accelerates ovarian cancer progression by regulating cell proliferation; Authors - Lin Xiu ; KD Validated
human	IF	Endocrinology; De novo-synthesized retinoic acid in ovarian antral follicles enhances FSH-mediated ovarian follicular cell differentiation and female fertility.; Authors - Tomoko Kawai
mouse	ChIP	Adv Sci (Weinh); Synthetic Retinoid Kills Drug-Resistant Cancer Stem Cells via Inducing RARγ-Translocation-Mediated Tension Reduction and Chromatin Decondensation; Authors - Yao Zhang