RARG Recombinant antibody

• 

  WB analysis using 86098-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86098-1-RR (RARG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RARG Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86098-1-RR

Clone No.250399C1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

NR1B3, RAR gamma, RARC, RAR-gamma, Retinoic acid receptor gamma

---

Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, NCCIT cells, T-47D cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86098-1-RR targets RARG in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RARG fusion protein Ag1991
• Full Name: retinoic acid receptor, gamma
• Calculated Molecular Weight: 454 aa, 50 kDa
• Observed Molecular Weight: 50-60kDa
• GenBank Accession Number: BC019098
• Gene Symbol: RARG
• Gene ID (NCBI): 5916
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13631
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Retinoic acid receptors (RARs) are nuclear hormone receptors that act as ligand-dependent transcriptional regulators. In their liganded state, RARs activate transcription, whereas in their nonliganded form, they repress transcription of their target genes. RARs have numerous target genes, which have retinoic response elements in their promoter regions (PMID:17574023). RARG is a member of the retinoid acid receptor (RAR) family, along with RARA, which is known to be involved PML/RARA fusion of acute promyelocytic leukemia, and RARB. and rearrangement that conferred to leukemic cells acute promyelocytic leukemia-like morphologic and immunophenotypic features (PMID:20935257).